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Infection and Immunity, October 2001, p. 6520-6522, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6520-6522.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Regulation of Streptococcus gordonii sspB by the sspA Gene Product

Azza El-Sabaeny,1,2 Donald R. Demuth,3 and Richard J. Lamont1,*

Department of Oral Biology, University of Washington, Seattle, Washington 981951; Department of Biochemistry, University of Pennsylvania, Philadelphia, Pennsylvania 191043; and Department of Botany and Microbiology, University of Alexandria, Alexandria, Egypt2

Received 12 February 2001/Returned for modification 17 April 2001/Accepted 10 July 2001

Streptococcus gordonii expresses two related adhesins, SspA and SspB, the genes for which are adjacent on the chromosome and are regulated independently. Although the adhesins are functionally similar, the sspA promoter is more active than that of sspB. In this study we show an additional role for SspA in the control of sspB activity. Gel shift and DNA footprinting assays demonstrate that the SspA protein binds to the sspB promoter and protects a region 233 to 264 bp upstream of the predicted -35 promoter element. The responsiveness of the sspB promoter to SspA was investigated with a promoter-cat reporter. Expression of the sspB promoter was reduced by over 60% in an SspA-deficient mutant of S. gordonii. These results indicate that expression of S. gordonii sspB is positively regulated by the sspA gene product.

* Corresponding author. Mailing address: Department of Oral Biology, P.O. Box 357132, University of Washington, Seattle, WA 98195-7132. Phone: (206) 616-9459. Fax: (206) 685-3162. E-mail: lamon{at}u.washington.edu.

Infection and Immunity, October 2001, p. 6520-6522, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6520-6522.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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