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Infection and Immunity, October 2001, p. 6558-6563, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6558-6563.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

DNA Immunization with Trypanosoma cruzi HSP70 Fused to the KMP11 Protein Elicits a Cytotoxic and Humoral Immune Response against the Antigen and Leads to Protection

Lourdes Planelles,1 M. Carmen Thomas,1 Carlos Alonso,2 and Manuel C. López1,*

Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina "López Neyra," CSIC, 18001 Granada,1 and Centro de Biología Molecular "Severo Ochoa," CSIC---UAM, 28049 Madrid,2 Spain

Received 23 April 2001/Returned for modification 5 June 2001/Accepted 7 July 2001

Murine immunization with Trypanosoma cruzi KMP11-HSP70 fused genes but not the KMP11 gene alone elicited both an immunoglobulin G2a long-lasting humoral immune response against KMP11 protein and activation of CD8+ cytotoxic T lymphocytes specific for two KMP11 peptides containing A2 motifs. Moreover, protection against the parasite challenge was observed after immunization with the chimeric gene.

* Corresponding author. Mailing address: Departamento de Biología Molecular, Instituto de Parasitología y Biomedicina "López Neyra," CSIC, Ventanilla 11, 18001 Granada, Spain. Phone: 34 958 203802. Fax: 34 958 203323. E-mail: mclopez{at}ipb.csic.es.

Infection and Immunity, October 2001, p. 6558-6563, Vol. 69, No. 10
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.10.6558-6563.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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