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Infection and Immunity, November 2001, p. 6749-6754, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6749-6754.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Analysis of Cytokines in the Early Development of Gastric Secondary Lymphoid Follicles in Helicobacter pylori-Infected BALB/c Mice with Neonatal Thymectomy

Kazushige Uchida,1 Kazuichi Okazaki,1,* Andras Debrecceni,1 Toshiki Nishi,1 Hirosi Iwano,1 Maki Inai,1 Suguru Uose,1 Hiroshi Nakase,1 Masaya Ohana,1 Chikashi Oshima,1 Yumi Matsushima,1 Chiharu Kawanami,1 Hiroshi Hiai,2 Toru Masuda,3 and Tsutomu Chiba1

Department of Gastroenterology and Endoscopic Medicine,1 Department of Pathology and Biology of Diseases,2 and Institute of Immunology,3 Graduate School of Medicine, Kyoto University, Kyoto, Japan

Received 29 September 2000/Returned for modification 8 February 2001/Accepted 10 July 2001

Immunological interaction between the host and Helicobacter pylori seems to play a critical role in follicular formation in gastric mucosa. We reported H. pylori-induced follicular gastritis model using neonatally thymectomized mice. In this study, we investigated the involvement of various cytokines in this model. BALB/c mice were thymectomized on the third day after birth (nTx). At 6 weeks old, these mice were orally infected with H. pylori. Histological studies showed that follicular formation occurred from 8 weeks after the infection and that most of the infiltrating lymphocytes were CD4+ and B cells. Neutrophils increased transiently at 1 week after the infection. Gamma interferon, interleukin-7 (IL-7), and IL-7 receptor were expressed in the stomach of the nTx mice irrespective of the infection. In contrast, expressions of the tumor necrosis factor alpha, IL-4 and lymphotoxin-alpha genes were remarkably upregulated by the infection. Our findings suggest that follicular formation may require cooperative involvement of a Th2-type immune response, tumor necrosis factor alpha and lymphotoxin-alpha in addition to the Th1-type immune response in H. pylori-induced gastritis in nTx mice.

* Corresponding author. Mailing address: Department of Gastroenterological Endoscopic Medicine, Postgraduate School of Medicine, Kyoto University, Shogoin-Kawaracho, Sakyo-ku, Kyoto City, Kyoto, 606-8507, Japan. Phone: 81-75-751-3413. Fax: 81-75-751-3414. E-mail: okak{at}kuhp.kyoto-u.ac.jp.

Infection and Immunity, November 2001, p. 6749-6754, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6749-6754.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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