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Infection and Immunity, November 2001, p. 6769-6775, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6769-6775.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Outer Membrane Targeting of Passenger Proteins by the Vacuolating Cytotoxin Autotransporter of Helicobacter pylori

Wolfgang Fischer,* Renate Buhrdorf, Elke Gerland,dagger and Rainer Haas

Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig-Maximilians-Universität, Munich, Germany

Received 16 April 2001/Returned for modification 22 May 2001/Accepted 18 July 2001

Helicobacter pylori produces a number of proteins associated with the outer membrane, including adhesins and the vacuolating cytotoxin. These proteins are supposed to integrate into the outer membrane by beta -barrel structures, characteristic of the family of autotransporter proteins. By using the SOMPES (shuttle vector-based outer membrane protein expression) system for outer membrane protein production, we were able to functionally express in H. pylori the cholera toxin B subunit genetically fused to the C-terminal VacA domain. We demonstrate that the fusion protein is translocated to the H. pylori outer membrane and that the CtxB domain is exposed on the H. pylori surface. Thus, we provide the first experimental evidence that the C-terminal beta -domain of VacA can transport a foreign passenger protein to the H. pylori surface and hence acts as a functional autotransporter.

* Corresponding author. Mailing address: Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Pettenkoferstr. 9a, D-80336 Munich, Germany. Phone: (49) 89-51605277. Fax: (49) 89-51605223. E-mail: schmitt{at}m3401.mpk.med.uni-muenchen.de.

dagger Present address: Creatogen AG, 86156 Augsburg, Germany.

Infection and Immunity, November 2001, p. 6769-6775, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6769-6775.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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