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Infection and Immunity, November 2001, p. 6831-6838, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6831-6838.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of the Hemolysis-Associated Protein 1 as a Cross-Protective Immunogen of Leptospira interrogans by Adenovirus-Mediated Vaccination

C. Branger,1 C. Sonrier,1 B. Chatrenet,2 B. Klonjkowski,3 N. Ruvoen-Clouet,1 A. Aubert,2 G. André-Fontaine,1,* and M. Eloit3

Unité de Bactériologie Médicale et Moléculaire des Leptospires, Ecole Nationale Vétérinaire de Nantes, 44307 Nantes Cedex 03,1 Virbac Laboratories, 06511 Carros Cedex,2 and UMR ENVA-INRA 955 de Génétique Moléculaire et Cellulaire, Génétique Virale, 94704 Maisons Alfort,3 France

Received 16 April 2001/Returned for modification 5 June 2001/Accepted 19 July 2001

New vaccine strategies are needed for the prevention of leptospirosis, a widespread human and animal disease caused by pathogenic leptospires. Our previous work determined that a protein leptospiral extract conferred cross-protection in a gerbil model of leptospirosis. The 31- to 34-kDa protein fraction of Leptospira interrogans serovar autumnalis was shown sufficient for this purpose. In the present study, N-terminal sequencing of a 32-kDa fraction and Southern blotting of genomic DNA with corresponding degenerated oligonucleotide probes identified two of its constituents: hemolysis-associated protein 1 (Hap1) and the outer membrane Leptospira protein 1 (OmpL1). Adenovirus-mediated Hap1 vaccination induces significant protection against a virulent heterologous Leptospira challenge in gerbils, whereas a similar OmpL1 construct failed to protect the animals. These data indicate that Hap1 could be a good candidate for developing a new generation of vaccines able to induce broad protection against leptospirosis disease.

* Corresponding author. Mailing address: Unité de Bactériologie Médicale et Moléculaire des Leptospires, Ecole Nationale Vétérinaire de Nantes, BP 40706, 44307 Nantes Cedex 03, France. Phone: 33 240 68 76 92. Fax: 33 240 68 76 94. E-mail: andre-fontaine{at}vet-nantes.fr.

Infection and Immunity, November 2001, p. 6831-6838, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6831-6838.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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