IAI FigSearch
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lima, O. C.
Right arrow Articles by Lopes Bezerra, L. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lima, O. C.
Right arrow Articles by Lopes Bezerra, L. M.

 Previous Article  |  Next Article 

Infection and Immunity, November 2001, p. 6874-6880, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6874-6880.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Involvement of Fungal Cell Wall Components in Adhesion of Sporothrix schenckii to Human Fibronectin

Osana C. Lima,1,2 Camila C. Figueiredo,1 José O. Previato,3 Lucia Mendonça-Previato,3 Verônica Morandi,1 and Leila M. Lopes Bezerra1,*

Departamento de Biologia Celular e Genética, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro,1 Fundação Oswaldo Cruz, Fiocruz,2 and Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro,3 Rio de Janeiro, RJ, Brazil

Received 26 March 2001/Returned for modification 9 May 2001/Accepted 9 August 2001

Systemic sporotrichosis is an emerging infection potentially fatal for immunocompromised patients. Adhesion to extracellular matrix proteins is thought to play a crucial role in invasive fungal diseases. Here we report studies of the adhesion of Sporothrix schenckii to the extracellular protein fibronectin (Fn). Both yeast cells and conidia of S. schenckii were able to adhere to Fn as detected by enzyme-linked immunosorbent binding assays. Adhesion of yeast cells to Fn is dose dependent and saturable. S. schenckii adheres equally well to 40-kDa and 120-kDa Fn proteolytic fragments. While adhesion to Fn was increased by Ca2+, inhibition assays demonstrated that it was not RGD dependent. A carbohydrate-containing cell wall neutral fraction blocked up to 30% of the observed adherence for the yeast cells. The biochemical nature of this fraction suggests the participation of cell surface glycoconjugates in binding by their carbohydrate or peptide moieties. These results provide new data concerning S. schenckii adhesion mechanisms, which could be important in host-fungus interactions and the establishment of sporotrichosis.

* Corresponding author. Mailing address: Departamento de Biologia Celular e Genética, Instituto de Biologia Roberto Alcântara Gomes, UERJ, Rua São Francisco Xavier, 524---PHLC s/205, 20550-013, Rio de Janeiro, RJ, Brazil. Phone: 55-21-2587-7567. Fax: 55-21-2587-7377. E-mail: leila{at}uerj.br.

Infection and Immunity, November 2001, p. 6874-6880, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6874-6880.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 2001 by the American Society for Microbiology. All rights reserved.