IAI FigSearch
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Solnick, J. V.
Right arrow Articles by Parsonnet, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Solnick, J. V.
Right arrow Articles by Parsonnet, J.

 Previous Article  |  Next Article 

Infection and Immunity, November 2001, p. 6887-6892, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6887-6892.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Determination of the Infectious Dose of Helicobacter pylori during Primary and Secondary Infection in Rhesus Monkeys (Macaca mulatta)

Jay V. Solnick,1,* Lori M. Hansen,1 Don R. Canfield,2 and Julie Parsonnet3

Departments of Internal Medicine and Medical Microbiology and Immunology, School of Medicine, University of California,1 and California Regional Primate Research Center,2 Davis, and Departments of Medicine and Health Research and Policy, Stanford University School of Medicine, Stanford,3 California

Received 16 February 2001/Returned for modification 7 June 2001/Accepted 24 July 2001

We sought to determine the infectious dose of Helicobacter pylori during primary and secondary infection in the rhesus monkey and to determine whether preinoculation acid suppression is necessary to produce colonization. Mixed inoculation with three human-derived strains showed that H. pylori J166 is particularly adapted to colonization of rhesus monkeys, since it outcompeted two other strains. The minimum infectious dose of H. pylori J166 was 104 bacteria in specific-pathogen (H. pylori)-free monkeys. Rechallenge of these monkeys after antibiotic therapy was characterized by a 10- to 100-fold decrease in bacterial load compared to primary infection, but with little change in the infectious dose. Acid suppression prior to inoculation was not necessary for colonization to occur. These results provide a basis for future animal experiments using more ecologically relevant conditions of inoculation and suggest that reduction in bacterial load rather than complete protection may be a more realistic goal for H. pylori vaccination.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, 3140 Tupper Hall, University of California, Davis, Davis, CA 95616. Phone: (530) 752-1333. Fax: (530) 752-8692. E-mail: jvsolnick{at}ucdavis.edu.

Infection and Immunity, November 2001, p. 6887-6892, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.6887-6892.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 2001 by the American Society for Microbiology. All rights reserved.