This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lai, Y.-C.
Right arrow Articles by Chang, H.-Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lai, Y.-C.
Right arrow Articles by Chang, H.-Y.

 Previous Article  |  Next Article 

Infection and Immunity, November 2001, p. 7140-7145, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.7140-7145.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Genes Induced In Vivo during Klebsiella pneumoniae CG43 Infection

Yi-Chyi Lai,1 Hwei-Ling Peng,2 and Hwan-You Chang1,*

Department of Life Science and Institute of Biotechnology, National Tsing Hua University,1 and Department of Biological Science and Technology, National Chiao Tung University,2 Hsin Chu, Taiwan, Republic of China

Received 16 January 2001/Returned for modification 20 March 2001/Accepted 20 July 2001

A novel in vivo expression technology (IVET) was performed to identify Klebsiella pneumoniae CG43 genes that are specifically expressed during infection of BALB/c mice. The IVET employed a UDP glucose pyrophosphorylase (galU)-deficient mutant of K. pneumoniae which is incapable of utilizing galactose and synthesizing capsular polysaccharide, as demonstrated by its low virulence to BALB/c mice and a white nonmucoid colony morphology on MacConkey-galactose agar. By using a functional galU gene as the reporter, an IVE promoter could render the galU mutant virulent while maintaining the white nonmucoid colony phenotype. A total of 20 distinct sequences were obtained through the in vivo selection. Five of them have been identified previously as virulence-associated genes in other pathogens, while another five with characterized functions are involved in regulation and transportation of nutrient uptake, biosynthesis of isoprenoids, and protein folding. No known functions have been attributed to the other 10 sequences. We have also demonstrated that 2 of the 20 IVE genes turn on under iron deprivation, whereas the expression of another five genes was found to be activated in the presence of paraquat, a superoxide generator.

* Corresponding author. Mailing address: Department of Life Science, National Tsing Hua University, 101 Kuan-Fu Road, 2nd Sec., Hsin Chu, Taiwan, Republic of China. Phone: 886-3-5742910. E-mail: hychang{at}life.nthu.edu.tw.

Infection and Immunity, November 2001, p. 7140-7145, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.7140-7145.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Begley, M., Bron, P. A., Heuston, S., Casey, P. G., Englert, N., Wiesner, J., Jomaa, H., Gahan, C. G. M., Hill, C. (2008). Analysis of the Isoprenoid Biosynthesis Pathways in Listeria monocytogenes Reveals a Role for the Alternative 2-C-Methyl-D-Erythritol 4-Phosphate Pathway in Murine Infection. Infect. Immun. 76: 5392-5401 [Abstract] [Full Text]  
  • Lawlor, M. S., O'Connor, C., Miller, V. L. (2007). Yersiniabactin Is a Virulence Factor for Klebsiella pneumoniae during Pulmonary Infection. Infect. Immun. 75: 1463-1472 [Abstract] [Full Text]  
  • Lin, C.-T., Peng, H.-L. (2006). Regulation of the Homologous Two-Component Systems KvgAS and KvhAS in Klebsiella pneumoniae CG43. J Biochem 140: 639-648 [Abstract] [Full Text]  
  • Lin, C.-T., Huang, T.-Y., Liang, W.-C., Peng, H.-L. (2006). Homologous Response Regulators KvgA, KvhA and KvhR Regulate the Synthesis of Capsular Polysaccharide in Klebsiella pneumoniae CG43 in a Coordinated Manner. J Biochem 140: 429-438 [Abstract] [Full Text]  
  • Boddicker, J. D., Anderson, R. A., Jagnow, J., Clegg, S. (2006). Signature-Tagged Mutagenesis of Klebsiella pneumoniae To Identify Genes That Influence Biofilm Formation on Extracellular Matrix Material.. Infect. Immun. 74: 4590-4597 [Abstract] [Full Text]  
  • Rediers, H., Rainey, P. B., Vanderleyden, J., De Mot, R. (2005). Unraveling the Secret Lives of Bacteria: Use of In Vivo Expression Technology and Differential Fluorescence Induction Promoter Traps as Tools for Exploring Niche-Specific Gene Expression. Microbiol. Mol. Biol. Rev. 69: 217-261 [Abstract] [Full Text]  
  • Silby, M. W., Levy, S. B. (2004). Use of In Vivo Expression Technology To Identify Genes Important in Growth and Survival of Pseudomonas fluorescens Pf0-1 in Soil: Discovery of Expressed Sequences with Novel Genetic Organization. J. Bacteriol. 186: 7411-7419 [Abstract] [Full Text]  
  • Bron, P. A., Grangette, C., Mercenier, A., de Vos, W. M., Kleerebezem, M. (2004). Identification of Lactobacillus plantarum Genes That Are Induced in the Gastrointestinal Tract of Mice. J. Bacteriol. 186: 5721-5729 [Abstract] [Full Text]  
  • Rediers, H., Bonnecarrere, V., Rainey, P. B., Hamonts, K., Vanderleyden, J., De Mot, R. (2003). Development and Application of a dapB-Based In Vivo Expression Technology System To Study Colonization of Rice by the Endophytic Nitrogen-Fixing Bacterium Pseudomonas stutzeri A15. Appl. Environ. Microbiol. 69: 6864-6874 [Abstract] [Full Text]  
  • Lai, Y.-C., Peng, H.-L., Chang, H.-Y. (2003). RmpA2, an Activator of Capsule Biosynthesis in Klebsiella pneumoniae CG43, Regulates K2 cps Gene Expression at the Transcriptional Level. J. Bacteriol. 185: 788-800 [Abstract] [Full Text]  
  • Struve, C., Forestier, C., Krogfelt, K. A. (2003). Application of a novel multi-screening signature-tagged mutagenesis assay for identification of Klebsiella pneumoniae genes essential in colonization and infection. Microbiology 149: 167-176 [Abstract] [Full Text]  
  • Angelichio, M. J., Camilli, A. (2002). In Vivo Expression Technology. Infect. Immun. 70: 6518-6523 [Full Text]  
  • Sarno, R., McGillivary, G., Sherratt, D. J., Actis, L. A., Tolmasky, M. E. (2002). Complete Nucleotide Sequence of Klebsiella pneumoniae Multiresistance Plasmid pJHCMW1. Antimicrob. Agents Chemother. 46: 3422-3427 [Abstract] [Full Text]  
  • Maroncle, N., Balestrino, D., Rich, C., Forestier, C. (2002). Identification of Klebsiella pneumoniae Genes Involved in Intestinal Colonization and Adhesion Using Signature-Tagged Mutagenesis. Infect. Immun. 70: 4729-4734 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»