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Infection and Immunity, November 2001, p. 7152-7158, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.7152-7158.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Inhibition of Attaching and Effacing Lesion Formation following Enteropathogenic Escherichia coli and Shiga Toxin-Producing E. coli Infection

Kathene Johnson-Henry,¹ John L. Wallace,² Naveen S. Basappa,¹ Rohini Soni,¹ Gilbert K. P. Wu,¹ and Philip M. Sherman^1,*

Research Institute, Hospital for Sick Children, Departments of Paediatrics and Laboratory Medicine & Pathobiology, University of Toronto, Toronto, Ontario,¹ and Department of Pharmacology, University of Calgary, Calgary, Alberta,² Canada

Received 18 January 2001/Returned for modification 16 March 2001/Accepted 1 August 2001

Enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) induce cytoskeletal changes in infected epithelial cells. To further characterize host cytosolic responses to infection, a series of specific cell-signaling inhibitors were employed. Initial bacterial adhesion to HEp-2 epithelial cells was not reduced, whereas -actinin accumulation in infected cells was blocked by a phosphoinositide-specific phospholipase C inhibitor (ET-18-OCH₃), phosphoinositide 3-kinase inhibitors (wortmannin and LY294002), and a 5-lipoxygenase inhibitor, nordihydroguaretic acid. A cyclooxygenase-2 inhibitor (NS-398), however, did not block -actinin reorganization in response to EPEC and STEC infections. Understanding signal transduction responses to enteric pathogens could provide the basis for the development of novel therapeutic strategies.

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^* Corresponding author. Mailing address: Division of Gastroenterology and Nutrition, Room 8409, Hospital for Sick Children, 555 University Ave., Toronto, Ontario M5G 1X8, Canada. Phone: (416) 813-7734. Fax: (416) 813-6531. E-mail: sherman{at}sickkids.on.ca.

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Infection and Immunity, November 2001, p. 7152-7158, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.7152-7158.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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