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Infection and Immunity, November 2001, p. 7187-7189, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.7187-7189.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Effect of SpeB and EndoS from Streptococcus pyogenes on Human Immunoglobulins

Mattias Collin* and Arne Olsén

Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, Lund, Sweden

Received 31 May 2001/Returned for modification 24 July 2001/Accepted 3 August 2001

Streptococcus pyogenes secretes a specific immunoglobulin G (IgG)-protease, SpeB, as well as the IgG glycan-hydrolyzing enzyme EndoS. Here we show that SpeB also degrades IgA, IgM, IgD, and IgE. We also show that EndoS only hydrolyzes the glycan moiety on native but not denatured IgG. Thus, SpeB has a broad immunoglobulin-degrading activity, while EndoS is highly specific for IgG.

* Corresponding author. Mailing address: Department of Cell and Molecular Biology, BMC-B14, SE-221 84, Lund, Sweden. Phone: 46(0)46-222 98 45. Fax: 46(0)46-15 77 56. E-mail: mattias.collin{at}medkem.lu.se.

Infection and Immunity, November 2001, p. 7187-7189, Vol. 69, No. 11
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.11.7187-7189.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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