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Infection and Immunity, December 2001, p. 7493-7500, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7493-7500.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Regulated Antigen Expression in Live Recombinant Salmonella enterica Serovar Typhimurium Strongly Affects Colonization Capabilities and Specific CD4+-T-Cell Responses

Dirk Bumann*

Max-Planck-Institut für Infektionsbiologie, Abteilung Molekulare Biologie, D-10117 Berlin, Germany

Received 18 June 2001/Returned for modification 26 July 2001/Accepted 4 September 2001

Regulated antigen expression can influence the immunogenicity of live recombinant Salmonella vaccines, but a rational optimization has remained difficult since important aspects of this effect are incompletely understood. Here, attenuated Salmonella enterica serovar Typhimurium SL3261 strains expressing the model antigen GFP_OVA were used to quantify in vivo antigen levels by flow cytometry and to simultaneously follow the crucial early steps of antigen-specific T-cell responses in mice that are transgenic for a T-cell receptor recognizing ovalbumin. Among seven tested promoters, PpagC has the highest activity in murine tissues combined with low in vitro expression, whereas Ptac has a comparable in vivo and a very high in vitro activity. Both SL3261 (pPpagCGFP_OVA) and SL3261 (pPtacGFP_OVA) cells can induce potent ovalbumin-specific cellular immune responses following oral administration, but doses almost 1,000-fold lower are sufficient for the in vivo-inducible construct SL3261 (pPpagCGFP_OVA) compared to SL3261 (pPtacGFP_OVA). This efficacy difference is largely explained by impaired early colonization capabilities of SL3261 (pPtacGFP_OVA) cells. Based on the findings of this study, appropriate in vivo expression levels for any given antigen can be rationally selected from the increasing set of promoters with defined properties. This will allow the improvement of recombinant Salmonella vaccines against a wide range of pathogens.

* Mailing address: Max-Planck-Institut für Infektionsbiologie, Abteilung Molekulare Biologie, Schumannstraße 21/22, D-10117 Berlin, Germany. Phone: 49 30 28460 430. Fax: 49 30 28460 401. E-mail: bumann{at}mpiib-berlin.mpg.de.

Infection and Immunity, December 2001, p. 7493-7500, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7493-7500.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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