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Infection and Immunity, December 2001, p. 7760-7771, Vol. 69, No. 12
Department of Microbiology, Hiroshima
University Faculty of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima
City, Hiroshima 734-8553,1 Department of
Microbiology, Miyazaki Medical College, Kiyotake 5200, Miyazaki
899-1692,2 Deep-Sea Microbial Genome
Research Group, Japan Marine Science and Technology Center, Natsushima
2-15, Yokosuka, 237-0061,3 and
Department of Bacteriology, Shinshu University School of
Medicine, Asahi 3-1-1, Matsumoto 390-8621,4
Japan
Received 25 May 2001/Returned for modification 16 August
2001/Accepted 10 September 2001
The complete nucleotide sequence of pETB, a 38.2-kb
Staphylococcus aureus plasmid encoding the exfoliative
toxin B (ETB), was determined. A total of 50 open reading frames were
identified on the plasmid genome and, among these, 32 showed sequence
similarity to known proteins. pETB contains three copies of
IS257, which divide the pETB genome into three regions:
(i) a cadmium resistance operon-containing region, (ii) a lantibiotic
production gene-containing region, and (iii) the remaining part where
genes for plasmid replication and/or maintenance are dispersed. In the
third region, genes of various kinds of functions are present among the
replication- and maintenance-related genes. They include two
virulence-related genes, the etb gene and a gene
encoding a novel ADP-ribosyltransferase closely related to EDIN, which
belongs to the C3 family of ADP-ribosyltransferases modifying Rho
GTPases. They also include genes for a cell wall-anchoring surface
protein and a phage resistance protein. Based on the determined sequence of pETB, the genome structures of etb-bearing
plasmids (ETB plasmids) from various clinical isolates were analyzed by the PCR scanning method. The data indicate that, although the ETB
plasmids are highly heterogeneous in genome size, the fundamental genome organization is well conserved. The size variation of the plasmid is mainly attributed to defined regions which may be hot spots
for gene shuffling.
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.12.7760-7771.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Complete Nucleotide Sequence of a
Staphylococcus aureus Exfoliative Toxin B Plasmid and
Identification of a Novel ADP-Ribosyltransferase, EDIN-C
*
Corresponding author. Mailing address: Department of
Microbiology, Hiroshima University Faculty of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima City, Hiroshima 734-8553, Japan. Phone: (81) 82-257-5635. Fax: (81) 82-257-5639. E-mail:
sugai{at}hiroshima-u.ac.jp.
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