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Infection and Immunity, December 2001, p. 7889-7893, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7889-7893.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Toxoplasma gondii Infection of Neurons Induces Neuronal Cytokine and Chemokine Production, but Gamma Interferon- and Tumor Necrosis Factor-Stimulated Neurons Fail To Inhibit the Invasion and Growth of T. gondii

D. Schlüter,1,* M. Deckert,2 H. Hof,1 and K. Frei3

Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Mannheim, Universität Heidelberg, Mannheim,1 and Abteilung für Neuropathologie, Universität zu Köln, Cologne,2 Germany, and Neurochirurgie, Universitätsspital Zürich, Zürich, Switzerland3

Received 10 April 2001/Returned for modification 29 May 2001/Accepted 27 August 2001

The intracellular parasite Toxoplasma gondii has the capacity to persist in the brain within neurons. In this study we demonstrated that T. gondii infected murine cerebellar neurons in vitro and replicated within these cells. Stimulation with gamma interferon (IFN-gamma ) and/or tumor necrosis factor (TNF) did not enable neurons to inhibit parasite invasion and replication. Cultured neurons constitutively produced interleukin 1 (IL-1), IL-6, macrophage inflammatory protein 1alpha (MIP-1alpha ), and MIP-1beta but not transforming growth factor beta 1 (TGF-beta 1), IL-10, and granulocyte-macrophage colony-stimulating factor. Neuronal expression of some cytokines (IL-6, TGF-beta 1) and chemokines (MIP-1beta ) was regulated by infection and/or by IFN-gamma and TNF.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Mannheim, Universität Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 Mannheim, Germany. Phone: (49) 621-383-2036. Fax: (49) 621-383-3886. E-mail: dirk.schlueter{at}imh.ma.uni-heidelberg.de.

Infection and Immunity, December 2001, p. 7889-7893, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7889-7893.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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