This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Singh, P.
Right arrow Articles by Datta, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Singh, P.
Right arrow Articles by Datta, A.

 Previous Article  |  Next Article 

Infection and Immunity, December 2001, p. 7898-7903, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7898-7903.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Attenuation of Virulence and Changes in Morphology in Candida albicans by Disruption of the N-Acetylglucosamine Catabolic Pathway

Praveen Singh, Sharmistha Ghosh, and Asis Datta*

School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India

Received 23 May 2001/Returned for modification 10 July 2001/Accepted 2 August 2001

A Candida albicans mutant with mutations in the N-acetylglucosamine (GlcNAc) catabolic pathway gene cluster, including the GlcNAc-6-phosphate deacetylase (DAC1), glucosamine-6-phosphate deaminase (NAG1), and GlcNAc kinase (HXK1) genes, was not able to grow on amino sugars, exhibited highly attenuated virulence in a murine systemic candidiasis model, and was less adherent to human buccal epithelial cells in vitro. No germ tubes were formed by the mutant after induction with GlcNAc, but the mutant exhibited hyperfilamentation under stress-induced filamentation conditions. In addition, the GlcNAc catabolic pathway played a vital role in determining the colony phenotype. Our results imply that this pathway is very important because of its diverse links with pathways involved in virulence and morphogenesis of the organism.

* Corresponding author. Mailing address: 105 Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Phone: 0091-11-616-2016, 0091-11-610-7676, and 0091-11-616-7557, ext. 2560 and 2001. Fax: 0091-11-619-8234. E-mail: adatta{at}jnuniv.ernet.in and asisdatta{at}hotmail.com.

Infection and Immunity, December 2001, p. 7898-7903, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7898-7903.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Biswas, S., Van Dijck, P., Datta, A. (2007). Environmental Sensing and Signal Transduction Pathways Regulating Morphopathogenic Determinants of Candida albicans. Microbiol. Mol. Biol. Rev. 71: 348-376 [Abstract] [Full Text]  
  • Alvarez, F. J., Konopka, J. B. (2007). Identification of an N-Acetylglucosamine Transporter That Mediates Hyphal Induction in Candida albicans. Mol. Biol. Cell 18: 965-975 [Abstract] [Full Text]  
  • Toenjes, K. A., Munsee, S. M., Ibrahim, A. S., Jeffrey, R., Edwards, J. E. Jr., Johnson, D. I. (2005). Small-Molecule Inhibitors of the Budded-to-Hyphal-Form Transition in the Pathogenic Yeast Candida albicans. Antimicrob. Agents Chemother. 49: 963-972 [Abstract] [Full Text]  
  • Brand, A., MacCallum, D. M., Brown, A. J. P., Gow, N. A. R., Odds, F. C. (2004). Ectopic Expression of URA3 Can Influence the Virulence Phenotypes and Proteome of Candida albicans but Can Be Overcome by Targeted Reintegration of URA3 at the RPS10 Locus. Eukaryot Cell 3: 900-909 [Abstract] [Full Text]  
  • vandenBerg, A. L., Ibrahim, A. S., Edwards, J. E. Jr., Toenjes, K. A., Johnson, D. I. (2004). Cdc42p GTPase Regulates the Budded-to-Hyphal-Form Transition and Expression of Hypha-Specific Transcripts in Candida albicans. Eukaryot Cell 3: 724-734 [Abstract] [Full Text]  
  • Biswas, S., Roy, M., Datta, A. (2003). N-Acetylglucosamine-inducible CaGAP1 encodes a general amino acid permease which co-ordinates external nitrogen source response and morphogenesis in Candida albicans. Microbiology 149: 2597-2608 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»