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Infection and Immunity, December 2001, p. 7898-7903, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7898-7903.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Attenuation of Virulence and Changes in Morphology in Candida albicans by Disruption of the N-Acetylglucosamine Catabolic Pathway

Praveen Singh, Sharmistha Ghosh, and Asis Datta*

School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India

Received 23 May 2001/Returned for modification 10 July 2001/Accepted 2 August 2001

A Candida albicans mutant with mutations in the N-acetylglucosamine (GlcNAc) catabolic pathway gene cluster, including the GlcNAc-6-phosphate deacetylase (DAC1), glucosamine-6-phosphate deaminase (NAG1), and GlcNAc kinase (HXK1) genes, was not able to grow on amino sugars, exhibited highly attenuated virulence in a murine systemic candidiasis model, and was less adherent to human buccal epithelial cells in vitro. No germ tubes were formed by the mutant after induction with GlcNAc, but the mutant exhibited hyperfilamentation under stress-induced filamentation conditions. In addition, the GlcNAc catabolic pathway played a vital role in determining the colony phenotype. Our results imply that this pathway is very important because of its diverse links with pathways involved in virulence and morphogenesis of the organism.

* Corresponding author. Mailing address: 105 Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India. Phone: 0091-11-616-2016, 0091-11-610-7676, and 0091-11-616-7557, ext. 2560 and 2001. Fax: 0091-11-619-8234. E-mail: adatta{at}jnuniv.ernet.in and asisdatta{at}hotmail.com.

Infection and Immunity, December 2001, p. 7898-7903, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7898-7903.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.