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Infection and Immunity, December 2001, p. 7927-7932, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7927-7932.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of Chlamydia pneumoniae Persistence in HEp-2 Cells Treated with Gamma Interferon

Laura G. Pantoja,1,2 Richard D. Miller,2 Julio A. Ramirez,1 Robert E. Molestina,1 and James T. Summersgill1,2,*

Division of Infectious Diseases, Department of Medicine,1 and Department of Microbiology and Immunology,2 University of Louisville School of Medicine, Louisville, Kentucky 40292

Received 27 March 2001/Returned for modification 8 June 2001/Accepted 23 August 2001

Infection with Chlamydia pneumoniae has been implicated as a potential risk factor for atherosclerosis. This study demonstrated the effects of gamma interferon (IFN-gamma )-mediated indoleamine 2,3-dioxygenase activity on C. pneumoniae persistence in HEp-2 cells, inclusion morphology, and ultrastructure. C. pneumoniae replication showed a dose-dependent decrease when treated with increasing concentrations of IFN-gamma and a phenotypic switch resulting in a decrease in typical inclusions with an increase in smaller, less-dense atypical inclusions. Ultrastructural analysis of IFN-gamma -treated C. pneumoniae revealed atypical inclusions containing large reticulatate-like aberrant bodies with no evidence of redifferentiation into elementary bodies.

* Corresponding author. Mailing address: Infectious Diseases Laboratory, Room 311, Instructional Building, 500 South Preston St., University of Louisville, Louisville, KY 40292. Phone: (502) 852-5132. Fax: (502) 852-1512. E-mail: jtsumm{at}louisville.edu.

Infection and Immunity, December 2001, p. 7927-7932, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7927-7932.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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