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Infection and Immunity, December 2001, p. 7933-7936, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7933-7936.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of the sigB Operon in Staphylococcus epidermidis: Construction and Characterization of a sigB Deletion Mutant

Stefanie Kies, Michael Otto,dagger Cuong Vuong,dagger and Friedrich Götz*

Mikrobielle Genetik, Universität Tübingen, 72076 Tübingen, Germany

Received 19 March 2001/Returned for modification 4 May 2001/Accepted 15 August 2001

The role of the alternative sigma factor sigma B in Staphylococcus epidermidis was investigated by the construction, complementation, and characterization of a sigB deletion mutant. Electrophoretic analyses confirmed a profound influence of sigma B on the expression of exoproteins and cytoplasmic proteins. Detailed investigation revealed reduced lipase and enhanced protease activity in the sigma B mutant. Furthermore, no significant influence of sigma B on heterologous biofilm formation or on the activity of the global regulator agr was detected.

* Corresponding author. Mailing address: Mikrobielle Genetik, Universität Tübingen, Waldhäuserstr. 70/8, 72076 Tübingen, Germany. Phone: 49 7071 2974636. Fax: 49 7071 295937. E-mail: friedrich.goetz{at}uni-tuebingen.de.

dagger Present address: Rocky Mountain Laboratory, Laboratory of Bacterial Pathogenesis, NIAID, NIH, Hamilton, MT 59840.

Infection and Immunity, December 2001, p. 7933-7936, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7933-7936.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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