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Infection and Immunity, December 2001, p. 7933-7936, Vol. 69, No. 12
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.12.7933-7936.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Identification of the sigB Operon in
Staphylococcus epidermidis: Construction and
Characterization of a sigB Deletion Mutant
Stefanie
Kies,
Michael
Otto,
Cuong
Vuong,
and
Friedrich
Götz*
Mikrobielle Genetik, Universität
Tübingen, 72076 Tübingen, Germany
Received 19 March 2001/Returned for modification 4 May
2001/Accepted 15 August 2001
The role of the alternative sigma factor
B in
Staphylococcus epidermidis was investigated by the
construction, complementation, and characterization of a
sigB deletion mutant. Electrophoretic analyses confirmed a
profound influence of
B on the expression of exoproteins
and cytoplasmic proteins. Detailed investigation revealed reduced
lipase and enhanced protease activity in the
B mutant.
Furthermore, no significant influence of
B on
heterologous biofilm formation or on the activity of the global regulator agr was detected.
*
Corresponding author. Mailing address:
Mikrobielle Genetik, Universität Tübingen,
Waldhäuserstr. 70/8, 72076 Tübingen, Germany. Phone: 49 7071 2974636. Fax: 49 7071 295937. E-mail: friedrich.goetz{at}uni-tuebingen.de.

Present address: Rocky Mountain Laboratory, Laboratory of Bacterial
Pathogenesis, NIAID, NIH, Hamilton, MT
59840.
Infection and Immunity, December 2001, p. 7933-7936, Vol. 69, No. 12
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.12.7933-7936.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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