Role of FliC and FliD Flagellar Proteins of Clostridium difficile in Adherence and Gut Colonization

doi:10.1128/IAI.69.12.7937-7940.2001

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Infection and Immunity, December 2001, p. 7937-7940, Vol. 69, No. 12
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.12.7937-7940.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role of FliC and FliD Flagellar Proteins of Clostridium difficile in Adherence and Gut Colonization

Albert Tasteyre, Marie-Claude Barc,^* Anne Collignon, Helene Boureau, and Tuomo Karjalainen

Département de Microbiologie, Faculté de Pharmacie, Université de Paris-Sud, 92296 Châtenay-Malabry Cedex, France

Received 16 April 2001/Returned for modification 27 June 2001/Accepted 11 September 2001

In vitro and in vivo adhesive properties of flagella and recombinant flagellin FliC and flagellar cap FliD proteins of Clostridiumdifficile were analyzed. FliC, FliD, and crude flagella adheredin vitro to axenic mouse cecal mucus. Radiolabeled cultured cellsbound to a high degree to FliD and weakly to flagella depositedon a membrane. The tissue association in the mouse cecum of anonflagellated strain was 10-fold lower than that of a flagellatedstrain belonging to the same serogroup, confirming the role offlagella inadherence.

^* Corresponding author. Mailing address: Université de Paris-Sud, Faculté de Pharmacie, Département de Microbiologie, 5,rue JB Clément, 92296 Châtenay-Malabry cedex, France. Phone:(33)-1 46 83 55 49. Fax: (33)-1 46 83 58 83. E-mail: marie-claude.barc{at}cep.u-psud.fr.

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