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Infection and Immunity, December 2001, p. 7950-7954, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7950-7954.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Salmonella DNA Adenine Methylase Mutants Elicit Protective Immune Responses to Homologous and Heterologous Serovars in Chickens

E. L. Dueger,1,2,* J. K. House,1,2 D. M. Heithoff,3 and M. J. Mahan3

Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 956161; Remedyne Corporation, Santa Barbara, California 931052; Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, California 931063

Received 26 June 2001/Returned for modification 4 September 2001/Accepted 21 September 2001

Salmonella DNA adenine methylase (Dam) mutants that lack or overproduce Dam are highly attenuated for virulence in mice and confer protection against murine typhoid fever. To determine whether vaccines based on Dam are efficacious in poultry, a Salmonella Dam- vaccine was evaluated in the protection of chicken broilers against oral challenge with homologous and heterologous Salmonella serovars. A Salmonella enterica serovar Typhimurium Dam- vaccine strain was attenuated for virulence in day-of-hatch chicks more than 100,000-fold. Vaccination of chicks elicited cross-protective immune responses, as evidenced by reduced colonization (10- to 10,000-fold) of the gastrointestinal tract (ileum, cecum, and feces) and visceral organs (bursa and spleen) after challenge with homologous (Typhimurium F98) and heterologous (Enteritidis 4973 and S. enterica O6,14,24: e,h-monophasic) Salmonella serovars that are implicated in Salmonella infection of poultry. The protection conferred was observed for the organ or the maximum CFU/tissue/bird as a unit of analysis, suggesting that Dam mutant strains may serve as the basis for the development of efficacious poultry vaccines for the containment of Salmonella.

* Corresponding author. Mailing address: Dept. of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616. Phone: (530) 752-7407. Fax: (530) 752-0414. E-mail: eldueger{at}ucdavis.edu.

Infection and Immunity, December 2001, p. 7950-7954, Vol. 69, No. 12
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.12.7950-7954.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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