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Infection and Immunity, February 2001, p. 1134-1141, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.1134-1141.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Schistosome Infection Stimulates Host CD4+ T Helper Cell and B-Cell Responses against a Novel Egg Antigen, Thioredoxin Peroxidase

David L. Williams,1,* Hiroko Asahi,2,dagger Douglas J. Botkin,1 and Miguel J. Stadecker2

Department of Biological Science, Illinois State University, Normal, Illinois 61790,1 and Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 021112

Received 17 August 2000/Returned for modification 11 October 2000/Accepted 2 November 2000

Egg granuloma formation during schistosome infections is mediated by CD4+ T helper (Th) cells sensitized to egg antigens; however, most of the relevant sensitizing egg antigens are still unknown. Here we show that schistosome thioredoxin peroxidase (TPx)-1 is a novel T- and B-cell egg antigen in schistosome-infected mice. CD4+ Th cell responses to fractionated egg components identified a significant response against a 26-kDa antigen; a partial amino acid sequence of this antigen was found to be identical to that of Schistosoma mansoni TPx-1. The native TPx-1 elicited significant proliferative responses as well as gamma interferon (IFN-gamma ), interleukin-2 (IL-2), IL-4, and IL-5 secretion in CD4+ cells from 8.5-week-infected CBA and C57BL/6 mice. By comparison, recombinant TPx-1 elicited a smaller, more type 1-polarized response, with significant production of IFN-gamma and IL-2, less IL-5, and essentially no IL-4. In C57BL/6 mice the responses to TPx-1 were relatively more prominent than that directed against the major egg antigen, Sm-p40, whereas in CBA mice the reverse was true. B-cell responses were also monitored in infected C57BL/6, C3H, CBA, and BALB/c mice. All strains had significant antibody levels against the TPx-1 protein, but the most significant antibody production ensued following parasite oviposition. TPx-1 was localized in eggs and shown to be secreted by eggs. The identification of egg antigens is important to understand the specific basis of granuloma formation in schistosome infections and may prove to be useful in strategies to ameliorate pathological responses.

* Corresponding author. Mailing address: Department of Biological Sciences. Illinois State University, Normal, IL 61761. Phone: (309) 438-2608. Fax: (309) 438-3722. E-mail: dlwilli{at}ilstu.edu.

dagger Present address: Department of Parasitology, NIID, Japan, Shinjuku-ku, Tokyo, Japan 162-8640.

Infection and Immunity, February 2001, p. 1134-1141, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.1134-1141.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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