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Infection and Immunity, February 2001, p. 1160-1171, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.1160-1171.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Allelic Variation within Helicobacter pylori babA and babB

David T. Pride,1,* Richard J. Meinersmann,2 and Martin J. Blaser1

Division of Infectious Diseases, Department of Medicine, Vanderbilt University School of Medicine and VA Medical Center, and Department of Microbiology and Immunology, Vanderbilt University, Nashville, Tennessee,1 and USDA Agricultural Research Service, Athens, Georgia2

Received 3 July 2000/Returned for modification 18 October 2000/Accepted 17 November 2000

Helicobacter pylori strains show both geographic and disease-associated allelic variation. We investigated the diversity present in two genes, babA and babB, which are members of a paralogous family of outer membrane proteins. Eleven family members within a single H. pylori strain, predicted to encode proteins with substantial N- and C-terminal similarity to each other, were classified as babA paralogues. In their central regions, most are less than 54% related to one another. Examining the babA and babB central regions in 42 H. pylori strains from different geographic locales, we identified five different allele groups of babA (AD1 to AD5) and three different allele groups of babB (BD1 to BD3). Phylogenetic analysis revealed that the allelic groupings of babA and babB are independent of one another and that, for both, geographic variation is present. Analysis of synonymous and nonsynonymous substitutions in these regions showed that babA is more diverse, implying an earlier origin than that of the same region of babB, but that the babA diversity region may have more functional constraints. Although recombination has been central to the evolution of both genes, with babA and babB showing low mean compatibility scores and homoplasy ratios of 0.71 and 0.67, respectively, recombination is not sufficient to obscure evidence of clonal descent. Despite the involvement of babA in binding to the host blood group antigen Lewis B, neither the presence of different babA allele groups nor that of different babB allele groups is a determining factor in Lewis B binding of H. pylori strains.

* Corresponding author. Mailing address: Department of Medicine, New York University School of Medicine, Infectious Diseases, Veterans Affairs Medical Center, Room 6006W, 423 East 23rd St., New York, NY 10010. Phone: (212) 252-7164. Fax: (212) 252-7167. E-mail: David.T.Pride{at}Vanderbilt.edu.

Infection and Immunity, February 2001, p. 1160-1171, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.1160-1171.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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