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Infection and Immunity, February 2001, p. 1160-1171, Vol. 69, No. 2
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.2.1160-1171.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Allelic Variation within Helicobacter pylori
babA and babB
David T.
Pride,1,*
Richard J.
Meinersmann,2 and
Martin J.
Blaser1
Division of Infectious Diseases, Department
of Medicine, Vanderbilt University School of Medicine and VA
Medical Center, and Department of Microbiology and Immunology,
Vanderbilt University, Nashville,
Tennessee,1 and USDA Agricultural
Research Service, Athens, Georgia2
Received 3 July 2000/Returned for modification 18 October
2000/Accepted 17 November 2000
Helicobacter pylori strains show both geographic and
disease-associated allelic variation. We investigated the diversity
present in two genes, babA and babB, which are
members of a paralogous family of outer membrane proteins. Eleven
family members within a single H. pylori strain, predicted
to encode proteins with substantial N- and C-terminal similarity to
each other, were classified as babA paralogues. In their
central regions, most are less than 54% related to one another.
Examining the babA and babB central regions in
42 H. pylori strains from different geographic locales, we
identified five different allele groups of babA (AD1 to
AD5) and three different allele groups of babB (BD1 to
BD3). Phylogenetic analysis revealed that the allelic groupings of
babA and babB are independent of one another
and that, for both, geographic variation is present. Analysis of
synonymous and nonsynonymous substitutions in these regions showed that
babA is more diverse, implying an earlier origin than that
of the same region of babB, but that the babA
diversity region may have more functional constraints. Although
recombination has been central to the evolution of both genes, with
babA and babB showing low mean compatibility
scores and homoplasy ratios of 0.71 and 0.67, respectively,
recombination is not sufficient to obscure evidence of clonal descent.
Despite the involvement of babA in binding to the host
blood group antigen Lewis B, neither the presence of different
babA allele groups nor that of different babB
allele groups is a determining factor in Lewis B binding of H. pylori strains.
*
Corresponding author. Mailing address: Department of
Medicine, New York University School of Medicine, Infectious Diseases, Veterans Affairs Medical Center, Room 6006W, 423 East 23rd St., New
York, NY 10010. Phone: (212) 252-7164. Fax: (212) 252-7167. E-mail:
David.T.Pride{at}Vanderbilt.edu.
Infection and Immunity, February 2001, p. 1160-1171, Vol. 69, No. 2
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.2.1160-1171.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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