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Infection and Immunity, February 2001, p. 1202-1206, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.1202-1206.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

DNA Immunization by Plasmodium falciparum Liver-Stage Antigen 3 Induces Protection against Plasmodium yoelii Sporozoite Challenge

Jean-Pierre Sauzet, Blanca-Liliana Perlaza, Karima Brahimi, Pierre Daubersies, and Pierre Druilhe*

Laboratoire de Parasitologie Médicale, Institut Pasteur, 75724 Paris Cedex 15, France

Received 21 August 2000/Returned for modification 26 September 2000/Accepted 6 November 2000

DNA-based immunization of mice by Plasmodium falciparum liver-stage antigen 3 (PfLSA3), a novel highly conserved P. falciparum preerythrocytic antigen, was evaluated. Animals developed a dominant Th1 immune response (high gamma interferon T-cell responses and predominance of immunoglobulin G2a) to each of three recombinant proteins spanning the molecule. We have exploited the immunological cross-reactivity of PfLSA3 with its putative homologue on sporozoites of the rodent parasite Plasmodium yoelii, and we show for the first time that responses induced by PfLSA3 in mice significantly protect against a heterologous challenge by P. yoelii sporozoites. These results support a significant effect of DNA-induced immune responses on preerythrocytic stages.

* Corresponding author. Mailing address: Laboratoire de Parasitologie Médicale, Institut Pasteur, 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 85 78. Fax: 33 1 45 68 86 40. E-mail: druilhe{at}pasteur.fr.

Infection and Immunity, February 2001, p. 1202-1206, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.1202-1206.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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