This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Diterich, I.
Right arrow Articles by Hartung, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Diterich, I.
Right arrow Articles by Hartung, T.

 Previous Article  |  Next Article 

Infection and Immunity, February 2001, p. 687-694, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.687-694.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Modulation of Cytokine Release in Ex Vivo-Stimulated Blood from Borreliosis Patients

Isabel Diterich,1 Luc Härter,1 Dieter Hassler,2 Albrecht Wendel,1 and Thomas Hartung1,*

Biochemical Pharmacology, Department of Biology, University of Konstanz, D-78457 Konstanz,1 and Untere Hofstatt 3, D-76703 Kraichtal,2 Germany

Received 26 July 2000/Returned for modification 20 September 2000/Accepted 23 October 2000

In lipopolysaccharide-stimulated blood from 71 late-stage borreliosis patients, the ex vivo cytokine release capacity of tumor necrosis factor alpha (TNF-alpha ) and gamma interferon (IFN-gamma ) was reduced to 28% ± 5% and to 31% ± 5% (P <=  0.001), respectively, compared to that of 24 healthy controls. White blood cell counts were normal in both groups. To investigate direct interactions between the pathogen and the immune cells, blood from healthy controls was exposed in vitro to live or heat-killed Borrelia or to Borrelia lysate. Compared to the pattern induced by bacterial endotoxins, a reduced release of TNF-alpha and IFN-gamma and an enhanced secretion of interleukin-10 and granulocyte colony-stimulating factor was found. In blood from 10 borreliosis patients stimulated with Borrelia lysate, TNF-alpha formation was decreased to 31% ± 14% and IFN-gamma formation was decreased to 8% ± 3% (P <=  0.001) compared to the cytokine response of blood from healthy controls (n = 24). We propose to consider anti-inflammatory changes in the blood cytokine response capacity elicited by Borrelia as a condition that might favor the persistence of the spirochete.

* Corresponding author. Mailing address: Biochemical Pharmacology, University of Konstanz, 78457 Konstanz, Germany. Phone: 49 7531 88 4116. Fax: 49 7531 88 4117. E-mail: Thomas.Hartung{at}uni-konstanz.de.

Infection and Immunity, February 2001, p. 687-694, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.687-694.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wang, G., Petzke, M. M., Iyer, R., Wu, H., Schwartz, I. (2008). Pattern of Proinflammatory Cytokine Induction in RAW264.7 Mouse Macrophages Is Identical for Virulent and Attenuated Borrelia burgdorferi. J. Immunol. 180: 8306-8315 [Abstract] [Full Text]  
  • Hermann, C. (2007). Review: Variability of host pathogen interaction. Innate Immunity 13: 199-218 [Abstract]  
  • Mueller, M., Bunk, S., Diterich, I., Weichel, M., Rauter, C., Hassler, D., Hermann, C., Crameri, R., Hartung, T. (2006). Identification of Borrelia burgdorferi Ribosomal Protein L25 by the Phage Surface Display Method and Evaluation of the Protein's Value for Serodiagnosis.. J. Clin. Microbiol. 44: 3778-3780 [Abstract] [Full Text]  
  • Mueller-Anneling, L. J., O'Neill, M. E., Thorne, P. S. (2006). Biomonitoring for assessment of organic dust-induced lung inflammation. Eur Respir J 27: 1096-1102 [Abstract] [Full Text]  
  • Rauter, C., Mueller, M., Diterich, I., Zeller, S., Hassler, D., Meergans, T., Hartung, T. (2005). Critical Evaluation of Urine-Based PCR Assay for Diagnosis of Lyme Borreliosis. CVI 12: 910-917 [Abstract] [Full Text]  
  • Salazar, J. C., Pope, C. D., Moore, M. W., Pope, J., Kiely, T. G., Radolf, J. D. (2005). Lipoprotein-Dependent and -Independent Immune Responses to Spirochetal Infection. CVI 12: 949-958 [Abstract] [Full Text]  
  • Diterich, I., Rauter, C., Kirschning, C. J., Hartung, T. (2003). Borrelia burgdorferi-Induced Tolerance as a Model of Persistence via Immunosuppression. Infect. Immun. 71: 3979-3987 [Abstract] [Full Text]  
  • Giambartolomei, G. H., Dennis, V. A., Lasater, B. L., Murthy, P. K., Philipp, M. T. (2002). Autocrine and Exocrine Regulation of Interleukin-10 Production in THP-1 Cells Stimulated with Borrelia burgdorferi Lipoproteins. Infect. Immun. 70: 1881-1888 [Abstract] [Full Text]  
  • Rauter, C., Oehme, R., Diterich, I., Engele, M., Hartung, T. (2002). Distribution of Clinically Relevant Borrelia Genospecies in Ticks Assessed by a Novel, Single-Run, Real-Time PCR. J. Clin. Microbiol. 40: 36-43 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»