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Infection and Immunity, February 2001, p. 737-743, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.737-743.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Salmonella Pathogenicity Island 2-Encoded Proteins SseC and SseD Are Essential for Virulence and Are Substrates of the Type III Secretion System

Joanna R. Klein and Bradley D. Jones*

Department of Microbiology, University of Iowa School of Medicine, Iowa City, Iowa 52242-1109

Received 28 August 2000/Returned for modification 18 October 2000/Accepted 8 November 2000

Survival of Salmonella enterica serovar Typhimurium within host phagocytic cells is a critical step in establishing systemic infection in mice. Genes within Salmonella pathogenicity island 2 (SPI-2) encode a type III secretion system that is required for establishment of systemic infection. Several proteins encoded by SPI-2 have homology to type III secreted proteins from enteropathogenic Escherichia coli and Yersinia and, based on that homology, are predicted to be secreted through the SPI-2 type III secretion system. We have investigated the roles of two of these proteins, SseC and SseD. We demonstrate here that the SseD protein is required for systemic Salmonella infection of the mouse, and we confirmed the virulence requirement for the SseC protein. Experiments were performed, using cellular fractionation and immunoblotting, to identify the subcellular location of the SseC and SseD proteins. Both proteins were found to localize predominantly to the bacterial cell membrane. In addition, our work revealed that SseC and SseD are exposed to the extracellular environment and are loosely associated with the bacterial membrane. Furthermore, localization of SseC and SseD to the bacterial membrane was found to require a functional SPI-2 type III secretion system. Collectively, these results indicate that the SseC and SseD proteins are secreted by the SPI-2 type III secretion system to the bacterial membrane in order to perform their virulence functions.

* Corresponding author. Mailing address: Department of Microbiology, University of Iowa College of Medicine, 3-330 Bowen Science Bldg., Iowa City, IA 52242. Phone: (319) 353-5457. Fax: (319) 335-9006. E-mail:bjones{at}blue.weeg.uiowa.edu.

Infection and Immunity, February 2001, p. 737-743, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.737-743.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.