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Infection and Immunity, February 2001, p. 800-809, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.800-809.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Fate of Mycobacterium tuberculosis within Murine Dendritic Cells

Kendra A. Bodnar, Natalya V. Serbina, and JoAnne L. Flynn*

Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261

Received 15 June 2000/Returned for modification 21 July 2000/Accepted 26 October 2000

The interaction of microbes with dendritic cells (DCs) is likely to have an enormous impact on the initiation of the immune response against a pathogen. In this study, we compared the interaction of Mycobacterium tuberculosis with murine bone marrow-derived DCs and macrophages (Mphi ) in vitro. M. tuberculosis grew equally well within nonactivated DCs and Mphi . Activation of DCs and Mphi with gamma interferon and lipopolysaccharide inhibited the growth of the intracellular bacteria in a nitric oxide synthase-dependent fashion. However, while this activation enabled Mphi to kill the intracellular bacteria, the M. tuberculosis bacilli within activated DCs were not killed. Thus, DCs could restrict the growth of the intracellular mycobacteria but were less efficient than Mphi at eliminating the infection. These results may have implications for priming immune responses to M. tuberculosis. In addition, they suggest that DCs may serve as a reservoir for M. tuberculosis in tissues, including the lymph nodes and lungs.

* Corresponding author. Mailing address: W1157 Biomedical Science Tower, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261. Phone: (412) 624-7743. Fax: (412) 624-1401. E-mail: joanne{at}pitt.edu.

Infection and Immunity, February 2001, p. 800-809, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.800-809.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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