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Infection and Immunity, February 2001, p. 800-809, Vol. 69, No. 2
Department of Molecular Genetics and
Biochemistry, University of Pittsburgh School of Medicine,
Pittsburgh, Pennsylvania 15261
Received 15 June 2000/Returned for modification 21 July
2000/Accepted 26 October 2000
The interaction of microbes with dendritic cells (DCs) is likely to
have an enormous impact on the initiation of the immune response
against a pathogen. In this study, we compared the interaction of
Mycobacterium tuberculosis with murine bone marrow-derived DCs and macrophages (M
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.2.800-809.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Fate of Mycobacterium tuberculosis within Murine
Dendritic Cells
) in vitro. M. tuberculosis grew
equally well within nonactivated DCs and M
. Activation of DCs and
M
with gamma interferon and lipopolysaccharide inhibited the growth of the intracellular bacteria in a nitric oxide synthase-dependent fashion. However, while this activation enabled M
to kill the intracellular bacteria, the M. tuberculosis bacilli within
activated DCs were not killed. Thus, DCs could restrict the growth of
the intracellular mycobacteria but were less efficient than M
at eliminating the infection. These results may have implications for
priming immune responses to M. tuberculosis. In addition, they suggest that DCs may serve as a reservoir for M. tuberculosis in tissues, including the lymph nodes and lungs.
*
Corresponding author. Mailing address: W1157 Biomedical
Science Tower, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261. Phone: (412) 624-7743. Fax: (412) 624-1401. E-mail: joanne{at}pitt.edu.
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