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Infection and Immunity, February 2001, p. 865-868, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.865-868.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Gene Discovery through Genomic Sequencing of Brucella abortus

Daniel O. Sánchez,1 Ruben O. Zandomeni,2 Silvio Cravero,3 Ramiro E. Verdún,1 Ester Pierrou,4 Paula Faccio,2 Gabriela Diaz,2 Silvia Lanzavecchia,2 Fernán Agüero,1 Alberto C. C. Frasch,1 Siv G. E. Andersson,4 Osvaldo L. Rossetti,3 Oscar Grau,2,5 and Rodolfo A. Ugalde1,*

Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús, CONICET/UNSAM, Universidad Nacional de General San Martín,1 Laboratorio de Alta Complejidad (IMYZA),2 and Instituto de Biotecnología, INTA,3 Buenos Aires, and IBBM, Universidad de la Plata, La Plata,5 Argentina, and Department of Molecular Evolution, Uppsala University, Uppsala, Sweden4

Received 29 June 2000/Returned for modification 29 August 2000/Accepted 6 November 2000

Brucella abortus is the etiological agent of brucellosis, a disease that affects bovines and human. We generated DNA random sequences from the genome of B. abortus strain 2308 in order to characterize molecular targets that might be useful for developing immunological or chemotherapeutic strategies against this pathogen. The partial sequencing of 1,899 clones allowed the identification of 1,199 genomic sequence surveys (GSSs) with high homology (BLAST expect value < 10-5) to sequences deposited in the GenBank databases. Among them, 925 represent putative novel genes for the Brucella genus. Out of 925 nonredundant GSSs, 470 were classified in 15 categories based on cellular function. Seven hundred GSSs showed no significant database matches and remain available for further studies in order to identify their function. A high number of GSSs with homology to Agrobacterium tumefaciens and Rhizobium meliloti proteins were observed, thus confirming their close phylogenetic relationship. Among them, several GSSs showed high similarity with genes related to nodule nitrogen fixation, synthesis of nod factors, nodulation protein symbiotic plasmid, and nodule bacteroid differentiation. We have also identified several B. abortus homologs of virulence and pathogenesis genes from other pathogens, including a homolog to both the Shda gene from Salmonella enterica serovar Typhimurium and the AidA-1 gene from Escherichia coli. Other GSSs displayed significant homologies to genes encoding components of the type III and type IV secretion machineries, suggesting that Brucella might also have an active type III secretion machinery.

* Corresponding author. Mailing address: Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, INTI (Ed. 24), Av. Gral Paz entre Constituyentes y Albarellos, 1650 San Martín, Provincia de Buenos Aires, Argentina. Phone: (54-11) 4580-7255. Fax: (54-11) 4752-9639. E-mail: rugalde{at}iib.unsam.edu.ar.

Infection and Immunity, February 2001, p. 865-868, Vol. 69, No. 2
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.2.865-868.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.