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Infection and Immunity, March 2001, p. 1306-1314, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1306-1314.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Canine Feces as a Reservoir of Extraintestinal Pathogenic Escherichia coli

James R. Johnson,* Adam L. Stell, and Parissa Delavaridagger

VA Medical Center and Department of Medicine, University of Minnesota, Minneapolis, Minnesota

Received 12 July 2000/Returned for modification 26 October 2000/Accepted 20 November 2000

To test the canine reservoir hypothesis of extraintestinal pathogenic Escherichia coli (ExPEC), 63 environmental canine fecal deposits were evaluated for the presence of ExPEC by a combination of selective culturing, extended virulence genotyping, hemagglutination testing, O serotyping, and PCR-based phylotyping. Overall, 30% of canine fecal samples (56% of those that yielded viable E. coli) contained papG-positive E. coli, usually as the predominant E. coli strain and always possessing papG allele III (which encodes variant III of the P-fimbrial adhesin molecule PapG). Multiple other virulence-associated genes typical of human ExPEC were prevalent among the canine fecal isolates. According to serotyping, virulence genotyping, and random amplified polymorphic DNA analysis, over 50% of papG-positive fecal E. coli could be directly correlated with specific human clinical isolates from patients with cystitis, pyelonephritis, bacteremia, or meningitis, including archetypal human ExPEC strains 536, CP9, and RS218. Five canine fecal isolates and (clonally related) archetypal human pyelonephritis isolate 536 were found to share a novel allele of papA (which encodes the P-fimbrial structural subunit PapA). These data confirm that ExPEC representing known virulent clones are highly prevalent in canine feces, which consequently may provide a reservoir of ExPEC for acquisition by humans.

* Corresponding author. Mailing address: Infectious Diseases (111F), VA Medical Center, 1 Veterans Dr., Minneapolis, MN 55417. Phone: (612) 725-2000, ext. 4185. Fax: (612) 725-2273. E-mail: johns007{at}tc.umn.edu.

dagger Present address: University of Minnesota Medical School, Minneapolis, Minn.

Infection and Immunity, March 2001, p. 1306-1314, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1306-1314.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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