This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bensing, B. A.
Right arrow Articles by Sullam, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bensing, B. A.
Right arrow Articles by Sullam, P. M.

 Previous Article  |  Next Article 

Infection and Immunity, March 2001, p. 1373-1380, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1373-1380.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genetic Loci of Streptococcus mitis That Mediate Binding to Human Platelets

Barbara A. Bensing,1 Craig E. Rubens,2 and Paul M. Sullam1,*

Veterans Affairs Medical Center and University of California San Francisco, San Francisco, California,1 and Children's Hospital, Seattle, Washington2

Received 1 September 2000/Returned for modification 3 November 2000/Accepted 27 November 2000

The direct binding of bacteria to platelets is a postulated major interaction in the pathogenesis of infective endocarditis. To identify bacterial components that mediate platelet binding by Streptococcus mitis, we screened a Tn916Delta E-derived mutant library of S. mitis strain SF100 for reduced binding to human platelets in vitro. Two distinct loci were found to affect platelet binding. The first contains a gene (pblT) encoding a highly hydrophobic, 43-kDa protein with 12 potential membrane-spanning segments. This protein resembles members of the major facilitator superfamily of small-molecule transporters. The second platelet binding locus consists of an apparent polycistronic operon. This region includes genes that are highly similar to those of Lactococcus lactis phage r1t and Streptococcus thermophilus phage 01205. Two genes (pblA and pblB) encoding large surface proteins are also present. The former encodes a 107-kDa protein containing tryptophan-rich repeats, which may serve to anchor the protein within the cell wall. The latter encodes a 121-kDa protein most similar to a tail fiber protein from phage 01205. Functional mapping by insertion-duplication mutagenesis and gene complementation indicates that PblB may be a platelet adhesin and that expression of PblB may be linked to that of PblA. The combined data indicate that at least two genomic regions contribute to platelet binding by S. mitis. One encodes a probable transmembrane transporter, while the second encodes two large surface proteins resembling structural components of lysogenic phages.

* Corresponding author. Mailing address: Division of Infectious Diseases, VA Medical Center (111W), 4150 Clement St., San Francisco, CA 94121. Phone: (415) 221-4810, ext. 2550. Fax: (415) 750-0502. E-mail: sullam{at}itsa.ucsf.edu.

Infection and Immunity, March 2001, p. 1373-1380, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1373-1380.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nobbs, A. H., Lamont, R. J., Jenkinson, H. F. (2009). Streptococcus Adherence and Colonization. Microbiol. Mol. Biol. Rev. 73: 407-450 [Abstract] [Full Text]  
  • Mitchell, J., Sullam, P. M. (2009). Streptococcus mitis Phage-Encoded Adhesins Mediate Attachment to {alpha}2-8-Linked Sialic Acid Residues on Platelet Membrane Gangliosides. Infect. Immun. 77: 3485-3490 [Abstract] [Full Text]  
  • Romero, P., Croucher, N. J., Hiller, N. L., Hu, F. Z., Ehrlich, G. D., Bentley, S. D., Garcia, E., Mitchell, T. J. (2009). Comparative Genomic Analysis of Ten Streptococcus pneumoniae Temperate Bacteriophages. J. Bacteriol. 191: 4854-4862 [Abstract] [Full Text]  
  • Zurla, C., Manzo, C., Dunlap, D., Lewis, D. E. A., Adhya, S., Finzi, L. (2009). Direct demonstration and quantification of long-range DNA looping by the {lambda} bacteriophage repressor. Nucleic Acids Res 37: 2789-2795 [Abstract] [Full Text]  
  • Loeffler, J. M., Fischetti, V. A. (2006). Lysogeny of Streptococcus pneumoniae with MM1 Phage: Improved Adherence and Other Phenotypic Changes.. Infect. Immun. 74: 4486-4495 [Abstract] [Full Text]  
  • Takamatsu, D., Bensing, B. A., Prakobphol, A., Fisher, S. J., Sullam, P. M. (2006). Binding of the Streptococcal Surface Glycoproteins GspB and Hsa to Human Salivary Proteins. Infect. Immun. 74: 1933-1940 [Abstract] [Full Text]  
  • Shen, K., Gladitz, J., Antalis, P., Dice, B., Janto, B., Keefe, R., Hayes, J., Ahmed, A., Dopico, R., Ehrlich, N., Jocz, J., Kropp, L., Yu, S., Nistico, L., Greenberg, D. P., Barbadora, K., Preston, R. A., Post, J. C., Ehrlich, G. D., Hu, F. Z. (2006). Characterization, Distribution, and Expression of Novel Genes among Eight Clinical Isolates of Streptococcus pneumoniae. Infect. Immun. 74: 321-330 [Abstract] [Full Text]  
  • Chia, J.-S., Lin, Y.-L., Lien, H.-T., Chen, J.-Y. (2004). Platelet Aggregation Induced by Serotype Polysaccharides from Streptococcus mutans. Infect. Immun. 72: 2605-2617 [Abstract] [Full Text]  
  • Siboo, I. R., Bensing, B. A., Sullam, P. M. (2003). Genomic Organization and Molecular Characterization of SM1, a Temperate Bacteriophage of Streptococcus mitis. J. Bacteriol. 185: 6968-6975 [Abstract] [Full Text]  
  • Tettelin, H., Masignani, V., Cieslewicz, M. J., Eisen, J. A., Peterson, S., Wessels, M. R., Paulsen, I. T., Nelson, K. E., Margarit, I., Read, T. D., Madoff, L. C., Wolf, A. M., Beanan, M. J., Brinkac, L. M., Daugherty, S. C., DeBoy, R. T., Durkin, A. S., Kolonay, J. F., Madupu, R., Lewis, M. R., Radune, D., Fedorova, N. B., Scanlan, D., Khouri, H., Mulligan, S., Carty, H. A., Cline, R. T., Van Aken, S. E., Gill, J., Scarselli, M., Mora, M., Iacobini, E. T., Brettoni, C., Galli, G., Mariani, M., Vegni, F., Maione, D., Rinaudo, D., Rappuoli, R., Telford, J. L., Kasper, D. L., Grandi, G., Fraser, C. M. (2002). Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae. Proc. Natl. Acad. Sci. USA 99: 12391-12396 [Abstract] [Full Text]  
  • Wagner, P. L., Waldor, M. K. (2002). Bacteriophage Control of Bacterial Virulence. Infect. Immun. 70: 3985-3993 [Full Text]  
  • Bensing, B. A., Siboo, I. R., Sullam, P. M. (2001). Proteins PblA and PblB of Streptococcus mitis, Which Promote Binding to Human Platelets, Are Encoded within a Lysogenic Bacteriophage. Infect. Immun. 69: 6186-6192 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»