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Infection and Immunity, March 2001, p. 1440-1443, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1440-1443.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Induction of Lysogenic Bacteriophage and Phage-Associated Toxin from Group A Streptococci during Coculture with Human Pharyngeal Cells

Thomas B. Broudy,* Vijaykumar Pancholi, and Vincent A. Fischetti

Department of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, New York 10021

Received 28 June 2000/Returned for modification 14 September 2000/Accepted 12 December 2000

We found that when group A streptococci are cocultured with human pharyngeal cells, they upregulate and secrete a 25-kDa toxin, determined to be the bacteriophage-encoded streptococcal pyrogenic exotoxin C (SpeC). This prompted us to determine if the bacteriophage themselves are induced during coculture conditions. We found that bacteriophage induction does occur, resulting in the release of ~105 phage particles during the 3-h coculture. Furthermore, we show that the bacteriophage induction event is mediated by a pharyngeal cell soluble factor for which we provide an initial characterization.

* Corresponding author. Mailing address: Department of Bacterial Pathogenesis and Immunology, Rockefeller University, 1230 York Ave., Box 172, New York, NY 10021. Phone: (212) 327-8167. Fax: (212) 327-7584. E-mail: broudyt{at}rockvax.rockefeller.edu.

Infection and Immunity, March 2001, p. 1440-1443, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1440-1443.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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