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Infection and Immunity, March 2001, p. 1625-1629, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1625-1629.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Implication of the Structure of the Helicobacter pylori cag Pathogenicity Island in Induction of Interleukin-8 Secretion

C. Audibert, C. Burucoa, B. Janvier, and J. L. Fauchère*

Laboratoire de Microbiologie A, CHU La Milétrie, 86021 Poitiers Cedex, France

Received 12 September 2000/Returned for modification 19 October 2000/Accepted 4 December 2000

Helicobacter pylori virulence is associated with the presence of the cag pathogenicity island (PAI). The cag PAI is involved in the ability to induce interleukin-8 (IL-8) secretion by human cells, which is implicated in the inflammatory response of the gastric mucosa to H. pylori infection. The aim of this study was to determine whether the genetic structure of the cag PAI is conserved and whether it is linked to IL-8 induction ability. Detection of specific markers (cagA, picB, cag13-cag14, virD4, and IS605) by PCR and dot blot hybridization and long-distance PCR determination of the presence of cagI, cagII, and the middle region of the cag PAI were performed on 153 strains isolated from adults suffering from ulcers (n = 79) or gastritis (n = 74). IL-8 induction ability was evaluated by coculture of the strains with HEp-2 cells. Eighty-three strains (54.3%) had an entire cag PAI, 12 strains (7.8%) had the cag PAI split in two, 49 strains (32%) had no cag PAI, and 9 strains exhibited other structural combinations. The presence of an entire cag PAI was statistically correlated with the presence of IS605 (P = 0.006) and the ability to induce IL-8 secretion but not with clinical presentation of the infection. The structure of the cag PAI appears to be rather conserved and is related to the proinflammatory power of a strain. The existence of strains inducing IL-8 secretion regardless of the cag PAI structure suggests that this region is not the only requirement for IL-8 secretion.

* Corresponding author. Mailing address: Laboratoire de Microbiologie A, CHU La Milétrie, BP 577, 86021 Poitiers cedex, France. Phone: 33 5 49 44 43 53. Fax: 33 5 49 44 38 88. E-mail: j.l.fauchere{at}chu-poitiers.fr.

Infection and Immunity, March 2001, p. 1625-1629, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1625-1629.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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