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Infection and Immunity, March 2001, p. 1708-1713, Vol. 69, No. 3
Department of Microbiology and Immunology,
University of Melbourne, Melbourne, Victoria 3010, Australia
Received 23 August 2000/Returned for modification 12 October
2000/Accepted 13 December 2000
Infection of different strains of mice with
Mycobacterium avium has revealed genetic control of the
immunoglobulin isotype induced and of the balance between Th1 and Th2
cytokines. Female BALB/c or C57BL/10 mice were infected intranasally
with 105 M. avium organisms. The antibody
response was measured over 18 weeks by enzyme-linked immunosorbent
assay and Western blotting, while numbers of cytokine-producing cells
were assessed at 12 to 15 weeks by ELISPOT assay. Upon infection,
C57BL/10 mice produced a clear Th1 response with strong gamma
interferon (IFN-
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.3.1708-1713.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Non-Major Histocompatibility Complex Control of
Antibody Isotype and Th1 versus Th2 Cytokines during Experimental
Infection of Mice with Mycobacterium avium
and
) production, no interleukin-4 (IL-4), and almost
entirely immunoglobulin G2a (IgG2a) antibody. In contrast, BALB/c mice
developed T cells producing IL-4, as well as those producing IFN-,
while the antibody response was a mixture of IgG1 and IgG2a. Antibodies
from BALB/c mice were also able to recognize a greater range of
antigens than were C56BL/10 mice. B10D2 mice, which carry the BALB/c
major histocompatibility complex haplotype on a C57BL/10 background,
followed the C57BL/10 cytokine pattern. Mice infected with
Listeria monocytogenes did not show a similar response dichotomy.
*
Corresponding author. Mailing address: Department of
Microbiology and Immunology, University of Melbourne, Melbourne,
Victoria 3010, Australia. Phone: 61 3 9344 5708. Fax: 61 3 8347 1560. E-mail: c.cheers{at}microbiology.unimelb.edu.au.
Present address: Division of Infectious Diseases, University of
California, Rosalind Russell Arthritis Research Laboratory and
Loewenstein Laboratory for Mycobacterial Research, San Francisco General Hospital, San Francisco, CA 94143.
Infection and Immunity, March 2001, p. 1708-1713, Vol. 69, No. 3
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.3.1708-1713.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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