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Infection and Immunity, March 2001, p. 1708-1713, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1708-1713.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Non-Major Histocompatibility Complex Control of Antibody Isotype and Th1 versus Th2 Cytokines during Experimental Infection of Mice with Mycobacterium avium

Vijaya Nagabhushanamdagger and Christina Cheers*

Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria 3010, Australia

Received 23 August 2000/Returned for modification 12 October 2000/Accepted 13 December 2000

Infection of different strains of mice with Mycobacterium avium has revealed genetic control of the immunoglobulin isotype induced and of the balance between Th1 and Th2 cytokines. Female BALB/c or C57BL/10 mice were infected intranasally with 105 M. avium organisms. The antibody response was measured over 18 weeks by enzyme-linked immunosorbent assay and Western blotting, while numbers of cytokine-producing cells were assessed at 12 to 15 weeks by ELISPOT assay. Upon infection, C57BL/10 mice produced a clear Th1 response with strong gamma interferon (IFN-gamma ) production, no interleukin-4 (IL-4), and almost entirely immunoglobulin G2a (IgG2a) antibody. In contrast, BALB/c mice developed T cells producing IL-4, as well as those producing IFN-gamma , while the antibody response was a mixture of IgG1 and IgG2a. Antibodies from BALB/c mice were also able to recognize a greater range of antigens than were C56BL/10 mice. B10D2 mice, which carry the BALB/c major histocompatibility complex haplotype on a C57BL/10 background, followed the C57BL/10 cytokine pattern. Mice infected with Listeria monocytogenes did not show a similar response dichotomy.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria 3010, Australia. Phone: 61 3 9344 5708. Fax: 61 3 8347 1560. E-mail: c.cheers{at}microbiology.unimelb.edu.au.

dagger Present address: Division of Infectious Diseases, University of California, Rosalind Russell Arthritis Research Laboratory and Loewenstein Laboratory for Mycobacterial Research, San Francisco General Hospital, San Francisco, CA 94143.

Infection and Immunity, March 2001, p. 1708-1713, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1708-1713.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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