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Infection and Immunity, March 2001, p. 1755-1765, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1755-1765.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Human Natural Killer Cells Mediate Killing of Intracellular Mycobacterium tuberculosis H37Rv via Granule-Independent Mechanisms

Kevin J. Brill,1 Qing Li,1 Rhonda Larkin,1 David H. Canaday,2,3 David R. Kaplan,4 W. Henry Boom,2,3 and Richard F. Silver1,2,3,*

Divisions of Pulmonary and Critical Care Medicine1 and Infectious Diseases,2 Department of Medicine, and Department of Pathology,4 Case Western Reserve University School of Medicine, and University Hospitals of Cleveland,3 Cleveland, Ohio 44106

Received 4 April 2000/Returned for modification 9 May 2000/Accepted 23 November 2000

Despite the continued importance of tuberculosis as a world-wide threat to public health, little is known about the mechanisms used by human lymphocytes to contain and kill the intracellular pathogen Mycobacterium tuberculosis. We previously described an in vitro model of infection of human monocytes (MN) with virulent M. tuberculosis strain H37Rv in which the ability of peripheral blood lymphocytes to limit intracellular growth of the organism could be measured. In the current study, we determined that lymphocyte-mediated killing of intracellular M. tuberculosis occurs within the first 24 h of coculture with infected MN. Natural killer (NK) cells isolated from both purified protein derivative (PPD)-positive and PPD-negative subjects were capable of mediating this early killing of intracellular H37Rv. NK cell-mediated killing of intracellular M. tuberculosis was not associated with the production of gamma interferon. Transferred supernatants of cocultured NK cells and M. tuberculosis-infected MN could not mediate the killing of intracellular M. tuberculosis, and Transwell studies indicated that direct cell-to-cell contact was required for NK cells to mediate the killing of the organism. Killing was not dependent upon exocytosis of NK cell cytotoxic granules. NK cells induced apoptosis of mycobacterium-infected MN, but neither killing of intracellular M. tuberculosis by NK cells nor NK cell-induced apoptosis of infected MN was inhibited by blocking the interaction of FasL and Fas. Thus, human NK cells may mediate killing of intracellular M. tuberculosis via alternative apoptotic pathways.

* Corresponding author. Mailing address: Divisions of Pulmonary and Critical Care Medicine and Infectious Diseases, Biomedical Research Bldg., Rm. 1030, Case Western Reserve University School of Medicine, 10900 Euclid Ave., Cleveland, OH 44106-4984. Phone: (216) 368-1151. Fax: (216) 368-2034. E-mail: rfs4{at}po.cwru.edu.

Infection and Immunity, March 2001, p. 1755-1765, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1755-1765.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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