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Infection and Immunity, March 2001, p. 1895-1901, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1895-1901.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role of Alginate O Acetylation in Resistance of Mucoid Pseudomonas aeruginosa to Opsonic Phagocytosis

Gerald B. Pier,1,* Fadie Coleman,1 Martha Grout,1 Michael Franklin,2 and Dennis E. Ohman3,4

Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115-58041; Department of Microbiology, Montana State University, Bozeman, Montana 597172; Department of Microbiology and Immunology, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, Virginia 23298-06783; and McGuire Veterans Affairs Medical Center, Richmond, Virginia 232494

Received 31 July 2000/Returned for modification 2 October 2000/Accepted 22 November 2000

Establishment and maintenance of chronic lung infections with mucoid Pseudomonas aeruginosa in patients with cystic fibrosis (CF) require that the bacteria avoid host defenses. Elaboration of the extracellular, O-acetylated mucoid exopolysaccharide, or alginate, is a major microbial factor in resistance to immune effectors. Here we show that O acetylation of alginate maximizes the resistance of mucoid P. aeruginosa to antibody-independent opsonic killing and is the molecular basis for the resistance of mucoid P. aeruginosa to normally nonopsonic but alginate-specific antibodies found in normal human sera and sera of infected CF patients. O acetylation of alginate appears to be critical for P. aeruginosa resistance to host immune effectors in CF patients.

* Corresponding author. Mailing address: Channing Laboratory, 181 Longwood Ave., Boston, MA 02115-5804. Phone: (617) 525 2269. Fax: (617) 731-1541. E-mail: gpier{at}channing.harvard.edu.

Infection and Immunity, March 2001, p. 1895-1901, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1895-1901.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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