Role of Alginate O Acetylation in Resistance of Mucoid Pseudomonas aeruginosa to Opsonic Phagocytosis

doi:10.1128/IAI.69.3.1895-1901.2001

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Infection and Immunity, March 2001, p. 1895-1901, Vol. 69, No. 3
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.3.1895-1901.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role of Alginate O Acetylation in Resistance of Mucoid Pseudomonas aeruginosa to Opsonic Phagocytosis

Gerald B. Pier,¹^,^* Fadie Coleman,¹ Martha Grout,¹ Michael Franklin,² and Dennis E. Ohman³^,⁴

Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115-5804¹; Department of Microbiology, Montana State University, Bozeman, Montana 59717²; Department of Microbiology and Immunology, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, Virginia 23298-0678³; and McGuire Veterans Affairs Medical Center, Richmond, Virginia 23249⁴

Received 31 July 2000/Returned for modification 2 October 2000/Accepted 22 November 2000

Establishment and maintenance of chronic lung infections with mucoid Pseudomonas aeruginosa in patients with cystic fibrosis(CF) require that the bacteria avoid host defenses. Elaborationof the extracellular, O-acetylated mucoid exopolysaccharide, oralginate, is a major microbial factor in resistance to immuneeffectors. Here we show that O acetylation of alginate maximizesthe resistance of mucoid P. aeruginosa to antibody-independentopsonic killing and is the molecular basis for the resistanceof mucoid P. aeruginosa to normally nonopsonic but alginate-specificantibodies found in normal human sera and sera of infected CFpatients. O acetylation of alginate appears to be critical forP. aeruginosa resistance to host immune effectors in CFpatients.

^* Corresponding author. Mailing address: Channing Laboratory, 181 Longwood Ave., Boston, MA 02115-5804. Phone: (617) 525 2269.Fax: (617) 731-1541. E-mail: gpier{at}channing.harvard.edu.

Infection and Immunity, March 2001, p. 1895-1901, Vol. 69, No. 3
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.3.1895-1901.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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