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Infection and Immunity, March 2001, p. 1917-1921, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1917-1921.2001

Novel Genetic and Phenotypic Heterogeneity in Bordetella bronchiseptica Pertactin

Karen B. Register*

Respiratory Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa 50010

Received 28 August 2000/Returned for modification 10 October 2000/Accepted 24 November 2000

The Bordetella bronchiseptica outer membrane protein pertactin is believed to function as an adhesin and is an important protective immunogen. Previous sequence analysis of the pertactin gene identified two regions predicted to encode amino acid repeat motifs. Recent studies have documented DNA sequence heterogeneity in both regions. The present study describes additional variants in these regions, which form the basis for six novel pertactin types. Immunoblotting demonstrated phenotypic heterogeneity in pertactin consistent with the predicted combined sizes of the repeat regions. A revised system for classifying B. bronchiseptica pertactin variants is proposed.

* Mailing address: Swine Respiratory Diseases Project, USDA/ARS/National Animal Disease Center, P.O. Box 70, 2300 Dayton Rd., Ames, IA 50010. Phone: (515) 663-7700. Fax: (515) 663-7458. E-mail: kregiste{at}nadc.ars.usda.gov.

Infection and Immunity, March 2001, p. 1917-1921, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1917-1921.2001


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