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Infection and Immunity, March 2001, p. 1929-1933, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1929-1933.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Legionella pneumophila Suppresses Interleukin-12 Production by Macrophages

Kazuto Matsunaga, Thomas W. Klein, Catherine Newton, Herman Friedman, and Yoshimasa Yamamoto*

Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa, Florida 33612

Received 8 September 2000/Returned for modification 6 October 2000/Accepted 1 December 2000

In vitro infection of macrophages with Legionella pneumophila induced interleukin-1alpha (IL-1alpha ), IL-10, monocyte chemotactic protein 1 (MCP-1), and MCP-3 but not IL-12. The lipopolysaccharide (LPS)-induced production of IL-12 was down-regulated by infection with virulent L. pneumophila, but other cytokines were not affected. In contrast, avirulent L. pneumophila or UV-killed, virulent L. pneumophila did not induce any suppression of IL-12. The IL-12 suppression occurred at the level of mRNA accumulation for IL-12 genes in response to LPS stimulation, but the infection induced a marked accumulation of mRNA for both MCP-1 and MCP-3, which are known to suppress IL-12 production in LPS-stimulated macrophages. However, pretreatment of macrophages with MCP-1 did not suppress LPS-induced IL-12 production at the concentrations induced by L. pneumophila infection. These results suggest that L. pneumophila selectively suppresses IL-12 production induced by LPS from macrophages in vitro by an MCP-independent mechanism.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, 12901 Bruce B. Downs Blvd., Tampa, FL 33612. Phone: (813) 974-2332. Fax: (813) 974-4151. E-mail: yyamamot{at}com1.med.usf.edu.

Infection and Immunity, March 2001, p. 1929-1933, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1929-1933.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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