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Infection and Immunity, March 2001, p. 1938-1942, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1938-1942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Expression of Cytolethal Distending Toxin and Hemolysin Is Not Required for Pustule Formation by Haemophilus ducreyi in Human Volunteers

Royden S. Young,1 Kate R. Fortney,1 Valentina Gelfanova,1 Carrie L. Phillips,2 Barry P. Katz,1 Antoinette F. Hood,1,3 Jo L. Latimer,4 Robert S. Munson Jr.,5,6 Eric J. Hansen,4 and Stanley M. Spinola1,2,7,*

Departments of Medicine,1 Microbiology and Immunology,7 Pathology and Laboratory Medicine,2 and Dermatology,3 School of Medicine, Indiana University, Indianapolis, Indiana 46202; Children's Research Institute5 and Departments of Pediatrics and Microbiology,6 The Ohio State University, Columbus, Ohio 43205-2696; and Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-90484

Received 27 September 2000/Returned for modification 8 November 2000/Accepted 24 November 2000

Haemophilus ducreyi makes cytolethal distending toxin (CDT) and hemolysin. In a previous human challenge trial, an isogenic hemolysin-deficient mutant caused pustules with a rate similar to that of its parent. To test whether CDT was required for pustule formation, six human subjects were inoculated with a CDT mutant and parent at multiple sites. The pustule formation rates were similar at both parent and mutant sites. A CDT and hemolysin double mutant was constructed and tested in five additional subjects. The pustule formation rates were similar for the parent and double mutant. These results indicate that neither the expression of CDT, nor that of hemolysin, nor both are required for pustule formation by H. ducreyi in humans.

* Corresponding author. Mailing address: Department of Medicine, 435 Emerson Hall, 545 Barnhill Dr., Indiana University, Indianapolis, IN 46202-5124. Phone: (317) 274-1427. Fax: (317) 274-1587. E-mail: sspinola{at}iupui.edu.

Infection and Immunity, March 2001, p. 1938-1942, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1938-1942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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