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Infection and Immunity, March 2001, p. 1961-1966, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1961-1966.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Selection of Virulence-Associated Determinants of Streptococcus suis Serotype 2 by In Vivo Complementation

Hilde E. Smith,1,* Herma Buijs,1 Henk J. Wisselink,1 Norbert Stockhofe-Zurwieden,2 and Mari A. Smits1

Department of Bacteriology1 and Department of Immunology, Pathology, and Epidemiology,2 Institute for Animal Science and Health, 8200 AB Lelystad, The Netherlands

Received 21 June 2000/Returned for modification 17 August 2000/Accepted 19 December 2000

Within Streptococcus suis serotype 2, pathogenic, weakly pathogenic, and nonpathogenic strains can be found. We introduced a genomic library of a pathogenic strain into a weakly pathogenic strain. After infection of the library into young piglets pathogenic transformants were selected. One specific transformant containing a 3-kb fragment of the pathogenic strain appeared to be dominantly enriched in diseased pigs. The observed enrichment was not tissue specific. The selected fragment, when introduced into two different weakly pathogenic strains, increased the virulence of these strains considerably. In contrast, introduction of the corresponding fragment of a weakly pathogenic strain had only minor effects on virulence. Nucleotide sequence analysis of the selected fragment of the pathogenic strain revealed the presence of two potential open reading frames, both of which were found to be mutated in the corresponding fragment of the weakly pathogenic strain. These data strongly suggest that the selected fragment contains determinants important for virulence.

* Corresponding author. Mailing address: Department of Bacteriology, Institute for Animal Science and Health, P.O. Box 65, 8200 AB Lelystad, The Netherlands. Phone: 31.320.238270. Fax: 31.320.238153. E-mail: h.e.smith{at}id.wag-ur.nl.

Infection and Immunity, March 2001, p. 1961-1966, Vol. 69, No. 3
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.3.1961-1966.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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  • Harel, J., Martinez, G., Nassar, A., Dezfulian, H., Labrie, S. J., Brousseau, R., Moineau, S., Gottschalk, M. (2003). Identification of an Inducible Bacteriophage in a Virulent Strain of Streptococcus suis Serotype 2. Infect. Immun. 71: 6104-6108 [Abstract] [Full Text]  
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