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Infection and Immunity, April 2001, p. 2092-2098, Vol. 69, No. 4
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.4.2092-2098.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Type II Protein Secretion Is a Subset of the
PilD-Dependent Processes That Facilitate Intracellular Infection by
Legionella pneumophila
Ombeline
Rossier and
Nicholas P.
Cianciotto*
Department of Microbiology and Immunology,
Northwestern University Medical School, Chicago, Illinois 60611
Received 27 October 2000/Returned for modification 1 December
2000/Accepted 20 December 2000
Previously, we had demonstrated that a Legionella
pneumophila prepilin peptidase (pilD) mutant does not
produce type IV pili and shows reduced secretion of enzymatic
activities. Moreover, it displays a distinct colony morphology and a
dramatic reduction in intracellular growth within amoebae and
macrophages, two phenotypes that are not exhibited by a pilin
(pilEL) mutant. To determine whether these
pilD-dependent defects were linked to type II secretion, we
have constructed two new mutants of L. pneumophila strain
130b. Mutations were introduced into either lspDE, which
encodes the type II outer membrane secretin and ATPase, or
lspFGHIJK, which encodes the pseudopilins. Unlike the
wild-type and pilEL strains, both
lspDE and lspG mutants showed reduced secretion
of six pilD-dependent enzymatic activities; i.e., protease,
acid phosphatase, p-nitrophenol phosphorylcholine
hydrolase, lipase, phospholipase A, and lysophospholipase A. However,
they exhibited a colony morphology different from that of the
pilD mutant, suggesting that their surfaces are distinct. The pilD, lspDE, and lspG mutants were
similarly and greatly impaired for growth within Hartmannella
vermiformis, indicating that the intracellular defect of the
peptidase mutant in amoebae is explained by the loss of type II
secretion. When assessed for infection of U937 macrophages, both
lsp mutants exhibited a 10-fold reduction in intracellular
multiplication and a diminished cytopathic effect. Interestingly, the
pilD mutant was clearly 100-fold more defective than the
type II secretion mutants in U937 cells. These results suggest the
existence of a novel pilD-dependent mechanism for promoting
L. pneumophila intracellular infection of human cells.
*
Corresponding author. Mailing address: Department of
Microbiology and Immunology, Northwestern University Medical School, 320 East Superior St., Chicago, IL 60611. Phone: (312) 503-0385. Fax:
(312) 503-1339. E-mail: n-cianciotto{at}northwestern.edu.
Infection and Immunity, April 2001, p. 2092-2098, Vol. 69, No. 4
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.4.2092-2098.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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