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Infection and Immunity, April 2001, p. 2107-2115, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2107-2115.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Complete Nucleotide Sequence and Analysis of the Locus of Enterocyte Effacement from Rabbit Diarrheagenic Escherichia coli RDEC-1

Chengru Zhu,1 Tonia S. Agin,1,dagger Simon J. Elliott,1,2,Dagger Laura A. Johnson,1 Timothy E. Thate,1 James B. Kaper,1,2 and Edgar C. Boedeker1,*

Center for Vaccine Development1 and Department of Microbiology and Immunology,2 University of Maryland School of Medicine, Baltimore, Maryland 21201

Received 7 July 2000/Returned for modification 14 September 2000/Accepted 1 December 2000

The pathogenicity island termed the locus of enterocyte effacement (LEE) is found in diverse attaching and effacing pathogens associated with diarrhea in humans and other animal species. To explore the relation of variation in LEE sequences to host specificity and genetic lineage, we determined the nucleotide sequence of the LEE region from a rabbit diarrheagenic Escherichia coli strain RDEC-1 (O15:H-) and compared it with those from human enteropathogenic E. coli (EPEC, O127:H6) and enterohemorrhagic E. coli (EHEC, O157:H7) strains. Differing from EPEC and EHEC LEEs, the RDEC-1 LEE is not inserted at selC and is flanked by an IS2 element and the lifA toxin gene. The RDEC-1 LEE contains a core region of 40 open reading frames, all of which are shared with the LEE of EPEC and EHEC. orf3 and the ERIC (enteric repetitive intergenic consensus) sequence present in the LEEs of EHEC and EPEC are absent from the RDEC-1 LEE. The predicted promoters of LEE1, LEE2, LEE3, tir, and LEE4 operons are highly conserved among the LEEs, although the upstream regions varied considerably for tir and the crucial LEE1 promoter, suggesting differences in regulation. Among the shared genes, high homology (>95% identity) between the RDEC-1 and the EPEC and EHEC LEEs at the predicted amino acid level was observed for the components of the type III secretion apparatus, the Ces chaperones, and the Ler regulator. In contrast, more divergence (66 to 88% identity) was observed in genes encoding proteins involved in host interaction, such as intimin (Eae) and the secreted proteins (Tir and Esps). A comparison of the highly variable genes from RDEC-1 with those from a number of attaching and effacing pathogens infecting different species and of different evolutionary lineages was performed. Although RDEC-1 diverges from some human-infecting EPEC and EHEC, most of the variation observed appeared to be due to evolutionary lineage rather than host specificity. Therefore, much of the observed hypervariability in genes involved in pathogenesis may not represent specific adaptation to different host species.

* Corresponding author. Mailing address: Center for Vaccine Development, University of Maryland School of Medicine, 685 West Baltimore St., Baltimore, MD 21201-1509. Phone: (410) 706-0330. Fax: (410) 706-6205. E-mail: eboedeke{at}medicine.umaryland.edu.

dagger Present address: Central Research Division, Pfizer Inc., Groton, CT 06340.

Dagger Present address: Department of Pediatric Infectious Disease, Johns Hopkins University, Baltimore, MD 21205.

Infection and Immunity, April 2001, p. 2107-2115, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2107-2115.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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