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Infection and Immunity, April 2001, p. 2172-2179, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2172-2179.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Effect of Mycobacterial Phospholipids on Interaction of Mycobacterium tuberculosis with Macrophages

Lisa M. Thorson,1,2 Daniel Doxsee,1,2 Monisha G. Scott,3 Paul Wheeler,4 and Richard W. Stokes1,2,3,5,*

Division of Infectious and Immunological Diseases, British Columbia's Children's Hospital,1 and Departments of Paediatrics,2 Microbiology & Immunology,3 and Pathology & Laboratory Medicine,5 University of British Columbia, Vancouver, British Columbia, Canada, and The Veterinary Laboratories Agency, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom4

Received 10 July 2000/Returned for modification 21 September 2000/Accepted 16 January 2001

This study demonstrates that pretreatment of macrophages with phosphatidylinositol, of either soya bean or mycobacterial origin, results in a down-regulation of the binding and uptake of Mycobacterium tuberculosis by the phagocytes. We also describe the novel observation that cardiolipin induces an increase in the binding and uptake of M. tuberculosis by macrophages. Neither phospholipid interacts with macrophages via the 2F8 epitope of scavenger receptor A, and treatment of macrophages with either phospholipid results in a down-regulation of CR3 function and tumor necrosis factor alpha production by the phagocyte. We have also shown that the ability of macrophages to interact with mycobacteria is greatly affected by an as yet unidentified product from the interaction of chloroform and polypropylene tubes.

* Corresponding author. Mailing address: Room 304, BC Research Institute for Children's and Women's Health, 950 West 28th Ave., Vancouver, BC V5Z 4H4, Canada. Phone: (604) 875-2466. Fax: (604) 875-2226. E-mail: rstokes{at}cbdn.ca.

Infection and Immunity, April 2001, p. 2172-2179, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2172-2179.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Villeneuve, C., Gilleron, M., Maridonneau-Parini, I., Daffe, M., Astarie-Dequeker, C., Etienne, G. (2005). Mycobacteria use their surface-exposed glycolipids to infect human macrophages through a receptor-dependent process. J. Lipid Res. 46: 475-483 [Abstract] [Full Text]  


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