This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rath, H. C.
Right arrow Articles by Sartor, R. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rath, H. C.
Right arrow Articles by Sartor, R. B.

 Previous Article  |  Next Article 

Infection and Immunity, April 2001, p. 2277-2285, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2277-2285.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Different Subsets of Enteric Bacteria Induce and Perpetuate Experimental Colitis in Rats and Mice

Heiko C. Rath,1,2 Michael Schultz,1,2 René Freitag,2 Levinus A. Dieleman,1 Fengling Li,1 Hans-Jörg Linde,3 Jürgen Schölmerich,2 and R. Balfour Sartor1,*

Center for GI Biology and Disease, University of North Carolina, Chapel Hill, North Carolina,1 and Department of Internal Medicine I2 and Institute of Medical Microbiology and Hygiene,3 University of Regensburg, Regensburg, Germany

Received 18 September 2000/Returned for modification 2 November 2000/Accepted 28 December 2000

Resident bacteria are incriminated in the pathogenesis of experimental colitis and inflammatory bowel diseases. We investigated the relative roles of various enteric bacteria populations in the induction and perpetuation of experimental colitis. HLA-B27 transgenic rats received antibiotics (ciprofloxacin, metronidazole, or vancomycin-imipenem) in drinking water or water alone in either prevention or treatment protocols. Mice were treated similarly with metronidazole or vancomycin-imipenem before or after receiving 5% dextran sodium sulfate (DSS). Germfree transgenic rats were colonized with specific-pathogen-free enteric bacteria grown overnight either in anaerobic or aerobic atmospheres. Nontransgenic rats colonized with anaerobic bacteria served as negative controls. Although preventive metronidazole significantly attenuated colitis in transgenic rats and DSS-treated mice, it had no therapeutic benefit once colitis was established. Ciprofloxacin also partially prevented but did not treat colitis in B27 transgenic rats. In both animal models vancomycin-imipenem most effectively prevented and treated colitis. Germfree transgenic rats reconstituted with enteric bacteria grown under anaerobic conditions had more aggressive colitis than those associated with aerobic bacteria. These results suggest that a subset of resident luminal bacteria induces colitis, but that a complex interaction of commensal aerobic and anaerobic bacteria provides the constant antigenic drive for chronic immune-mediated colonic inflammation.

* Corresponding author. Mailing address: Division of Digestive Diseases, CB#7038, Room 032A Glaxo Bldg., University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7038. Phone: (919) 966-0149. Fax: (919) 966-7468. E-mail: rbs{at}med.unc.edu.

Infection and Immunity, April 2001, p. 2277-2285, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2277-2285.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chen, G. Y., Shaw, M. H., Redondo, G., Nunez, G. (2008). The Innate Immune Receptor Nod1 Protects the Intestine from Inflammation-Induced Tumorigenesis. Cancer Res. 68: 10060-10067 [Abstract] [Full Text]  
  • Chichlowski, M., Hale, L. P. (2008). Bacterial-mucosal interactions in inflammatory bowel disease--an alliance gone bad. Am. J. Physiol. Gastrointest. Liver Physiol. 295: G1139-G1149 [Abstract] [Full Text]  
  • van der Kleij, H., O'Mahony, C., Shanahan, F., O'Mahony, L., Bienenstock, J. (2008). Protective effects of Lactobacillus reuteri and Bifidobacterium infantis in murine models for colitis do not involve the vagus nerve. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295: R1131-R1137 [Abstract] [Full Text]  
  • Gareau, M. G, Jury, J., MacQueen, G., Sherman, P. M, Perdue, M. H (2007). Probiotic treatment of rat pups normalises corticosterone release and ameliorates colonic dysfunction induced by maternal separation. Gut 56: 1522-1528 [Abstract] [Full Text]  
  • Dotan, I., Allez, M., Nakazawa, A., Brimnes, J., Schulder-Katz, M., Mayer, L. (2007). Intestinal epithelial cells from inflammatory bowel disease patients preferentially stimulate CD4+ T cells to proliferate and secrete interferon-{gamma}. Am. J. Physiol. Gastrointest. Liver Physiol. 292: G1630-G1640 [Abstract] [Full Text]  
  • Heimesaat, M. M., Bereswill, S., Fischer, A., Fuchs, D., Struck, D., Niebergall, J., Jahn, H.-K., Dunay, I. R., Moter, A., Gescher, D. M., Schumann, R. R., Gobel, U. B., Liesenfeld, O. (2006). Gram-Negative Bacteria Aggravate Murine Small Intestinal Th1-Type Immunopathology following Oral Infection with Toxoplasma gondii. J. Immunol. 177: 8785-8795 [Abstract] [Full Text]  
  • Medina, C., Radomski, M. W. (2006). Role of Matrix Metalloproteinases in Intestinal Inflammation. J. Pharmacol. Exp. Ther. 318: 933-938 [Abstract] [Full Text]  
  • Strauch, U G, Obermeier, F, Grunwald, N, Gurster, S, Dunger, N, Schultz, M, Griese, D P, Mahler, M, Scholmerich, J, Rath, H C (2005). Influence of intestinal bacteria on induction of regulatory T cells: lessons from a transfer model of colitis. Gut 54: 1546-1552 [Abstract] [Full Text]  
  • Stremmel, W, Merle, U, Zahn, A, Autschbach, F, Hinz, U, Ehehalt, R (2005). Retarded release phosphatidylcholine benefits patients with chronic active ulcerative colitis. Gut 54: 966-971 [Abstract] [Full Text]  
  • Fukata, M., Michelsen, K. S., Eri, R., Thomas, L. S., Hu, B., Lukasek, K., Nast, C. C., Lechago, J., Xu, R., Naiki, Y., Soliman, A., Arditi, M., Abreu, M. T. (2005). Toll-like receptor-4 is required for intestinal response to epithelial injury and limiting bacterial translocation in a murine model of acute colitis. Am. J. Physiol. Gastrointest. Liver Physiol. 288: G1055-G1065 [Abstract] [Full Text]  
  • Kieper, W. C., Troy, A., Burghardt, J. T., Ramsey, C., Lee, J. Y., Jiang, H.-Q., Dummer, W., Shen, H., Cebra, J. J., Surh, C. D. (2005). Cutting Edge: Recent Immune Status Determines the Source of Antigens That Drive Homeostatic T Cell Expansion. J. Immunol. 174: 3158-3163 [Abstract] [Full Text]  
  • Maeda, S., Hsu, L.-C., Liu, H., Bankston, L. A., Iimura, M., Kagnoff, M. F., Eckmann, L., Karin, M. (2005). Nod2 Mutation in Crohn's Disease Potentiates NF-{kappa}B Activity and IL-1{beta} Processing. Science 307: 734-738 [Abstract] [Full Text]  
  • Rachinel, N., Buzoni-Gatel, D., Dutta, C., Mennechet, F. J. D., Luangsay, S., Minns, L. A., Grigg, M. E., Tomavo, S., Boothroyd, J. C., Kasper, L. H. (2004). The Induction of Acute Ileitis by a Single Microbial Antigen of Toxoplasma gondii. J. Immunol. 173: 2725-2735 [Abstract] [Full Text]  
  • Schultz, M., Munro, K., Tannock, G. W., Melchner, I., Gottl, C., Schwietz, H., Scholmerich, J., Rath, H. C. (2004). Effects of Feeding a Probiotic Preparation (SIM) Containing Inulin on the Severity of Colitis and on the Composition of the Intestinal Microflora in HLA-B27 Transgenic Rats. CVI 11: 581-587 [Abstract] [Full Text]  
  • Schultz, M., Strauch, U. G., Linde, H.-J., Watzl, S., Obermeier, F., Gottl, C., Dunger, N., Grunwald, N., Scholmerich, J., Rath, H. C. (2004). Preventive Effects of Escherichia coli Strain Nissle 1917 on Acute and Chronic Intestinal Inflammation in Two Different Murine Models of Colitis. CVI 11: 372-378 [Abstract] [Full Text]  
  • Furrie, E, Macfarlane, S, Cummings, J H, Macfarlane, G T (2004). Systemic antibodies towards mucosal bacteria in ulcerative colitis and Crohn's disease differentially activate the innate immune response. Gut 53: 91-98 [Abstract] [Full Text]  
  • Alyanakian, M.-A., You, S., Damotte, D., Gouarin, C., Esling, A., Garcia, C., Havouis, S., Chatenoud, L., Bach, J.-F. (2003). Diversity of regulatory CD4+T cells controlling distinct organ-specific autoimmune diseases. Proc. Natl. Acad. Sci. USA 100: 15806-15811 [Abstract] [Full Text]  
  • Hoentjen, F, Harmsen, H J M, Braat, H, Torrice, C D, Mann, B A, Sartor, R B, Dieleman, L A (2003). Antibiotics with a selective aerobic or anaerobic spectrum have different therapeutic activities in various regions of the colon in interleukin 10 gene deficient mice. Gut 52: 1721-1727 [Abstract] [Full Text]  
  • Pavan, S., Desreumaux, P., Mercenier, A. (2003). Use of Mouse Models To Evaluate the Persistence, Safety, and Immune Modulation Capacities of Lactic Acid Bacteria. CVI 10: 696-701 [Abstract] [Full Text]  
  • Boisse, L., Sickle, M. D V., Sharkey, K. A, Pittman, Q. J (2003). Compromised neuroimmune status in rats with experimental colitis. J. Physiol. 548: 929-939 [Abstract] [Full Text]  
  • Ortega-Cava, C. F., Ishihara, S., Rumi, M. A. K., Kawashima, K., Ishimura, N., Kazumori, H., Udagawa, J., Kadowaki, Y., Kinoshita, Y. (2003). Strategic Compartmentalization of Toll-Like Receptor 4 in the Mouse Gut. J. Immunol. 170: 3977-3985 [Abstract] [Full Text]  
  • Dieleman, L A, Goerres, M S, Arends, A, Sprengers, D, Torrice, C, Hoentjen, F, Grenther, W B, Sartor, R B (2003). Lactobacillus GG prevents recurrence of colitis in HLA-B27 transgenic rats after antibiotic treatment. Gut 52: 370-376 [Abstract] [Full Text]  
  • Melmed, G., Thomas, L. S., Lee, N., Tesfay, S. Y., Lukasek, K., Michelsen, K. S., Zhou, Y., Hu, B., Arditi, M., Abreu, M. T. (2003). Human Intestinal Epithelial Cells Are Broadly Unresponsive to Toll-Like Receptor 2-Dependent Bacterial Ligands: Implications for Host-Microbial Interactions in the Gut. J. Immunol. 170: 1406-1415 [Abstract] [Full Text]  
  • Wei, B., Huang, T., Dalwadi, H., Sutton, C. L., Bruckner, D., Braun, J. (2002). Pseudomonas fluorescens Encodes the Crohn's Disease-Associated I2 Sequence and T-Cell Superantigen. Infect. Immun. 70: 6567-6575 [Abstract] [Full Text]  
  • Bamias, G., Marini, M., Moskaluk, C. A., Odashima, M., Ross, W. G., Rivera-Nieves, J., Cominelli, F. (2002). Down-Regulation of Intestinal Lymphocyte Activation and Th1 Cytokine Production by Antibiotic Therapy in a Murine Model of Crohn's Disease. J. Immunol. 169: 5308-5314 [Abstract] [Full Text]  
  • Podolsky, D. K. (2002). Inflammatory Bowel Disease. NEJM 347: 417-429 [Full Text]  
  • Iqbal, N., Oliver, J. R., Wagner, F. H., Lazenby, A. S., Elson, C. O., Weaver, C. T. (2002). T Helper 1 and T Helper 2 Cells Are Pathogenic in an Antigen-specific Model of Colitis. JEM 195: 71-84 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»