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Infection and Immunity, April 2001, p. 2383-2389, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2383-2389.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Expression of Chlamydia pneumoniae Polymorphic Membrane Protein Family Genes

Jane Grimwood,1,dagger Lynn Olinger,1 and Richard S. Stephens1,2,*

Francis I. Proctor Foundation, University of California, San Francisco,1 and Division of Infectious Diseases, School of Public Health, University of California, Berkeley,2 California

Received 17 October 2000/Returned for modification 7 December 2000/Accepted 8 January 2001

The genome of the obligate intracellular bacterium Chlamydia pneumoniae CWL029 encodes a family of 21 proteins with predicted outer membrane localization. These polymorphic membrane proteins (Pmps) are heterogeneous in both amino acid sequence and predicted size but are unified by the conserved amino acid motifs GGAI and FXXN repeated in the N-terminal half of each protein. Reverse transcriptase PCR analysis showed that all pmp genes are transcribed. To determine whether all proteins are expressed, specific antisera were generated by immunization with mutually exclusive synthetic peptides representing each of the 21 predicted Pmps. Each antiserum reacted with, and was typically immunospecific for, the corresponding peptide immunogen by enzyme-linked immunosorbent assay. Western blot analyses of purified elementary bodies showed that 11 of the 21 Pmps were detectable. Attempts to demonstrate by Sarykosyl fractionation that the Pmps were localized to the outer membrane revealed that several of the Pmps were unstable and readily degraded. Analyses of additional C. pneumoniae strains showed that although some Pmps are conserved, others vary between strains, in both molecular weight and level of expression.

* Corresponding author. Mailing address: Division of Infectious Diseases, School of Public Health, 235 Earl Warren Hall, University of California, Berkeley, CA 94720-7360. Phone: (510) 643-9900. Fax: (510) 643-1537. E-mail: rss{at}uclink4.berkeley.edu.

dagger Present address: Stanford Human Genome Center, Palo Alto, CA 94304.

Infection and Immunity, April 2001, p. 2383-2389, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2383-2389.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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