This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chung, J. Y.
Right arrow Articles by Adler, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chung, J. Y.
Right arrow Articles by Adler, B.

 Previous Article  |  Next Article 

Infection and Immunity, April 2001, p. 2487-2492, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2487-2492.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role of Capsule in the Pathogenesis of Fowl Cholera Caused by Pasteurella multocida Serogroup A

Jing Yeng Chung,1 Ian Wilkie,2 John D. Boyce,1 Kirsty M. Townsend,2 Alan J. Frost,2 Majid Ghoddusi,2 and Ben Adler1,*

Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800,1 and School of Veterinary Science and Animal Production, University of Queensland, Queensland 4072,2 Australia

Received 16 November 2000/Returned for modification 3 January 2001/Accepted 11 January 2001

We have constructed a defined acapsular mutant in Pasteurella multocida X-73 (serogroup A:1) by disrupting the hexA gene through the insertion of a tetracycline resistance cassette. The genotype of the hexA::tet(M) strain was confirmed by PCR and Southern hybridization, and the acapsular phenotype of this strain was confirmed by electron microscopy. The hexA::tet(M) strain was attenuated in both mice and chickens. Complementation of the mutant with an intact hexAB fragment restored lethality in mice but not in chickens. In contrast to the results described previously for P. multocida serogroup B (J. D. Boyce and B. Adler, Infect. Immun. 68:3463-3468, 2000), the hexA::tet(M) strain was sensitive to the bactericidal action of chicken serum, whereas the wild-type and complemented strains were both resistant. Following inoculation into chicken muscle, the bacterial count of the hexA::tet(M) strain decreased significantly, while the wild-type and complemented strains both grew rapidly over 4 h. The capsule is thus an essential virulence determinant in the pathogenesis of fowl cholera.

* Corresponding author. Mailing address: Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800, Australia. Phone: 61 3 9905 4815. Fax: 61 3 9905 4811. E-mail: Ben.Adler{at}med.monash.edu.au.

Infection and Immunity, April 2001, p. 2487-2492, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2487-2492.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • St. Michael, F., Harper, M., Parnas, H., John, M., Stupak, J., Vinogradov, E., Adler, B., Boyce, J. D., Cox, A. D. (2009). Structural and Genetic Basis for the Serological Differentiation of Pasteurella multocida Heddleston Serotypes 2 and 5. J. Bacteriol. 191: 6950-6959 [Abstract] [Full Text]  
  • Tang, X., Zhao, Z., Hu, J., Wu, B., Cai, X., He, Q., Chen, H. (2009). Isolation, Antimicrobial Resistance, and Virulence Genes of Pasteurella multocida Strains from Swine in China. J. Clin. Microbiol. 47: 951-958 [Abstract] [Full Text]  
  • Weigel, P. H., DeAngelis, P. L. (2007). Hyaluronan Synthases: A Decade-plus of Novel Glycosyltransferases. J. Biol. Chem. 282: 36777-36781 [Abstract] [Full Text]  
  • Harper, M., Cox, A., St. Michael, F., Parnas, H., Wilkie, I., Blackall, P. J., Adler, B., Boyce, J. D. (2007). Decoration of Pasteurella multocida Lipopolysaccharide with Phosphocholine Is Important for Virulence. J. Bacteriol. 189: 7384-7391 [Abstract] [Full Text]  
  • Tatum, F. M., Briggs, R. E. (2005). Construction of In-Frame aroA Deletion Mutants of Mannheimia haemolytica, Pasteurella multocida, and Haemophilus somnus by Using a New Temperature-Sensitive Plasmid. Appl. Environ. Microbiol. 71: 7196-7202 [Abstract] [Full Text]  
  • Hundt, M. J., Ruffolo, C. G. (2005). Interaction of Pasteurella multocida with Free-Living Amoebae. Appl. Environ. Microbiol. 71: 5458-5464 [Abstract] [Full Text]  
  • Urick, T., I-Chang, C., Arena, E., Xu, W., Bessman, M. J., Ruffolo, C. G. (2005). The pnhA Gene of Pasteurella multocida Encodes a Dinucleoside Oligophosphate Pyrophosphatase Member of the Nudix Hydrolase Superfamily. J. Bacteriol. 187: 5809-5817 [Abstract] [Full Text]  
  • Boucher, D. J., Adler, B., Boyce, J. D. (2005). The Pasteurella multocida nrfE Gene Is Upregulated during Infection and Is Essential for Nitrite Reduction but Not for Virulence. J. Bacteriol. 187: 2278-2285 [Abstract] [Full Text]  
  • Steenbergen, S. M., Lichtensteiger, C. A., Caughlan, R., Garfinkle, J., Fuller, T. E., Vimr, E. R. (2005). Sialic Acid Metabolism and Systemic Pasteurellosis. Infect. Immun. 73: 1284-1294 [Abstract] [Full Text]  
  • Catry, B., Chiers, K., Schwarz, S., Kehrenberg, C., Decostere, A., de Kruif, A. (2005). Fatal Peritonitis Caused by Pasteurella multocida Capsular Type F in Calves. J. Clin. Microbiol. 43: 1480-1483 [Abstract] [Full Text]  
  • Harper, M., Cox, A. D., St. Michael, F., Wilkie, I. W., Boyce, J. D., Adler, B. (2004). A Heptosyltransferase Mutant of Pasteurella multocida Produces a Truncated Lipopolysaccharide Structure and Is Attenuated in Virulence. Infect. Immun. 72: 3436-3443 [Abstract] [Full Text]  
  • Harper, M., Boyce, J. D., Wilkie, I. W., Adler, B. (2003). Signature-Tagged Mutagenesis of Pasteurella multocida Identifies Mutants Displaying Differential Virulence Characteristics in Mice and Chickens. Infect. Immun. 71: 5440-5446 [Abstract] [Full Text]  
  • Chen, L., Paulsen, D. B., Scruggs, D. W., Banes, M. M., Reeks, B. Y., Lawrence, M. L. (2003). Alteration of DNA adenine methylase (Dam) activity in Pasteurella multocida causes increased spontaneous mutation frequency and attenuation in mice. Microbiology 149: 2283-2290 [Abstract] [Full Text]  
  • Bandara, A. B., Lawrence, M. L., Veit, H. P., Inzana, T. J. (2003). Association of Actinobacillus pleuropneumoniae Capsular Polysaccharide with Virulence in Pigs. Infect. Immun. 71: 3320-3328 [Abstract] [Full Text]  
  • McKerral, L. J., Lo, R. Y. C. (2002). Construction and Characterization of an Acapsular Mutant of Mannheimia haemolytica A1. Infect. Immun. 70: 2622-2629 [Abstract] [Full Text]  
  • DeAngelis, P. L. (2002). Microbial glycosaminoglycan glycosyltransferases. Glycobiology 12: 9R-16R [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»