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Infection and Immunity, April 2001, p. 2487-2492, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2487-2492.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role of Capsule in the Pathogenesis of Fowl Cholera Caused by Pasteurella multocida Serogroup A

Jing Yeng Chung,1 Ian Wilkie,2 John D. Boyce,1 Kirsty M. Townsend,2 Alan J. Frost,2 Majid Ghoddusi,2 and Ben Adler1,*

Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800,1 and School of Veterinary Science and Animal Production, University of Queensland, Queensland 4072,2 Australia

Received 16 November 2000/Returned for modification 3 January 2001/Accepted 11 January 2001

We have constructed a defined acapsular mutant in Pasteurella multocida X-73 (serogroup A:1) by disrupting the hexA gene through the insertion of a tetracycline resistance cassette. The genotype of the hexA::tet(M) strain was confirmed by PCR and Southern hybridization, and the acapsular phenotype of this strain was confirmed by electron microscopy. The hexA::tet(M) strain was attenuated in both mice and chickens. Complementation of the mutant with an intact hexAB fragment restored lethality in mice but not in chickens. In contrast to the results described previously for P. multocida serogroup B (J. D. Boyce and B. Adler, Infect. Immun. 68:3463-3468, 2000), the hexA::tet(M) strain was sensitive to the bactericidal action of chicken serum, whereas the wild-type and complemented strains were both resistant. Following inoculation into chicken muscle, the bacterial count of the hexA::tet(M) strain decreased significantly, while the wild-type and complemented strains both grew rapidly over 4 h. The capsule is thus an essential virulence determinant in the pathogenesis of fowl cholera.


* Corresponding author. Mailing address: Bacterial Pathogenesis Research Group, Department of Microbiology, Monash University, Victoria 3800, Australia. Phone: 61 3 9905 4815. Fax: 61 3 9905 4811. E-mail: Ben.Adler{at}med.monash.edu.au.


Infection and Immunity, April 2001, p. 2487-2492, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2487-2492.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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