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Infection and Immunity, April 2001, p. 2580-2588, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2580-2588.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mu-Like Prophage in Serogroup B Neisseria meningitidis Coding for Surface-Exposed Antigens

Vega Masignani,1 Marzia Monica Giuliani,1 Hervé Tettelin,2 Maurizio Comanducci,1 Rino Rappuoli,1 and Vincenzo Scarlato1,3,*

Department of Molecular Biology, IRIS, Chiron S.p.A., 53100 Siena,1 and Department of Biology, University of Bologna, 40126 Bologna,3 Italy, and The Institute for Genomic Research, Rockville, Maryland 208502

Received 9 October 2000/Returned for modification 4 December 2000/Accepted 20 December 2000

Sequence analysis of the genome of Neisseria meningititdis serogroup B revealed the presence of an ~35-kb region inserted within a putative gene coding for an ABC-type transporter. The region contains 46 open reading frames, 29 of which are colinear and homologous to the genes of Escherichia coli Mu phage. Two prophages with similar organizations were also found in serogroup A meningococcus, and one was found in Haemophilus influenzae. Early and late phage functions are well preserved in this family of Mu-like prophages. Several regions of atypical nucleotide content were identified. These likely represent genes acquired by horizontal transfer. Three of the acquired genes are shown to code for surface-associated antigens, and the encoded proteins are able to induce bactericidal antibodies.

* Corresponding author. Mailing address: IRIS, Chiron S.p.A., Via Fiorentina 1, 53100 Siena, Italy. Phone: 39 0577 243565. Fax: 39 0577 243564. E-mail: enzo_scarlato{at}chiron.it.

Infection and Immunity, April 2001, p. 2580-2588, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2580-2588.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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