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Infection and Immunity, April 2001, p. 2732-2735, Vol. 69, No. 4
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.4.2732-2735.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Generation and Characterization of a Defined Mutant
of Streptococcus suis Lacking Suilysin
Andrew G.
Allen,1,
Steven
Bolitho,1
Heather
Lindsay,1
Shahid
Khan,1
Clare
Bryant,1
Pam
Norton,2
Philip
Ward,2
James
Leigh,2
Jeremy
Morgan,2
Helen
Riches,2
Sarah
Eastty,2 and
Duncan
Maskell1,*
Centre for Veterinary Science, Department of
Clinical Veterinary Medicine, University of Cambridge, Cambridge
CB3 0ES,1 and Institute for Animal
Health, Compton, Newbury, Berkshire RG20 7NN,2
United Kingdom
Received 24 July 2000/Returned for modification 29 August
2000/Accepted 2 January 2001
A defined allelic-replacement mutant of the sly gene,
encoding a thiol-activated cytolysin, from a European isolate of
Streptococcus suis serotype 2 was generated and
characterized. Unlike the parental strain, it is nonhemolytic,
noncytotoxic for cultured macrophage-like cells, avirulent in a mouse
infection model, yet only slightly attenuated in a porcine model of
systemic infection.
*
Corresponding author. Mailing address: Centre for
Veterinary Science, Department of Clinical Veterinary Medicine,
University of Cambridge, Madingley Rd., Cambridge, CB3 0ES, United
Kingdom. Phone: 44 1223 339868. Fax: 44 1223 337610. E-mail:
djm47{at}cam.ac.uk.

Present address: Arrow Therapeutics Ltd., Carshalton, Surrey,
SM5 4DS, United
Kingdom.
Infection and Immunity, April 2001, p. 2732-2735, Vol. 69, No. 4
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.4.2732-2735.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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