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Infection and Immunity, April 2001, p. 2732-2735, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2732-2735.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Generation and Characterization of a Defined Mutant of Streptococcus suis Lacking Suilysin

Andrew G. Allen,1,dagger Steven Bolitho,1 Heather Lindsay,1 Shahid Khan,1 Clare Bryant,1 Pam Norton,2 Philip Ward,2 James Leigh,2 Jeremy Morgan,2 Helen Riches,2 Sarah Eastty,2 and Duncan Maskell1,*

Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES,1 and Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN,2 United Kingdom

Received 24 July 2000/Returned for modification 29 August 2000/Accepted 2 January 2001

A defined allelic-replacement mutant of the sly gene, encoding a thiol-activated cytolysin, from a European isolate of Streptococcus suis serotype 2 was generated and characterized. Unlike the parental strain, it is nonhemolytic, noncytotoxic for cultured macrophage-like cells, avirulent in a mouse infection model, yet only slightly attenuated in a porcine model of systemic infection.

* Corresponding author. Mailing address: Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Rd., Cambridge, CB3 0ES, United Kingdom. Phone: 44 1223 339868. Fax: 44 1223 337610. E-mail: djm47{at}cam.ac.uk.

dagger Present address: Arrow Therapeutics Ltd., Carshalton, Surrey, SM5 4DS, United Kingdom.

Infection and Immunity, April 2001, p. 2732-2735, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2732-2735.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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