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Infection and Immunity, April 2001, p. 2739-2742, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2739-2742.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Borrelia burgdorferi Proteins Whose Expression Is Similarly Affected by Culture Temperature and pH

Ramesh Ramamoorthy* and Dorothy Scholl-Meeker

Department of Parasitology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 70433

Received 5 October 2000/Returned for modification 10 November 2000/Accepted 9 January 2001

Previously, we had demonstrated the upregulation in the expression of several proteins, including the lipoproteins OspC and P35, of Borrelia burgdorferi in the stationary growth phase. Since the expression of OspC is also known to be affected by culture temperature and pH, we examined the effects of both variables on the expression of the remaining stationary-phase-upregulated proteins. Our study revealed that the expression of each of the remaining stationary-phase-upregulated proteins, P35 included, was also influenced by culture temperature; these proteins were selectively expressed at 34°C but not at 24°C. Significantly, the expression of a majority of these proteins was also affected by culture pH, since they were abundantly expressed at pH 7.0 (resembling the tick midgut pH of 6.8 during feeding) but only sparsely at pH 8.0 (a condition closer to that of the unfed tick midgut pH of 7.4). We propose that this group of B. burgdorferi proteins, which in culture is selectively expressed under conditions of 34°C and pH 7.0, may be induced in the tick midgut during the feeding event. Furthermore, the differential and coordinate expression of these proteins under different environmental conditions suggests that the encoding genes may be coregulated.

* Corresponding author. Mailing address: TRPRC, 18703 Three Rivers Rd., Covington, LA 70433. Phone: (504) 892-2040, ext. 6361. Fax: (504) 893-1352. E-mail: moorthy{at}tpc.tulane.edu.

Infection and Immunity, April 2001, p. 2739-2742, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2739-2742.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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