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Infection and Immunity, April 2001, p. 2748-2752, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2748-2752.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of the Exported Proteins of the Oral Opportunistic Pathogen Actinobacillus actinomycetemcomitans by Using Alkaline Phosphatase Fusions

John Ward,1,* Julie Fletcher,2 Sean P. Nair,3 Michael Wilson,2 Rachel J. Williams,3 Stephen Poole,4 and Brian Henderson3

Molecular Microbiology Group, Department of Biochemistry and Molecular Biology, University College London, London WC1E 6BT,1 Microbiology Department2 and Cellular Microbiology Research Group,3 Eastman Dental Institute, University College London, London WC1X 8LD, and Division of Endocrinology, National Institute for Standards and Control, Potter's Bar EN6 3QG,4 United Kingdom

Received 23 August 2000/Returned for modification 30 October 2000/Accepted 3 January 2001

A phoA fusion library of Actinobacillus actinomycetemcomitans genomic DNA has been screened to identify genes encoding exported and secreted proteins. A total of 8,000 colonies were screened, and 80 positive colonies were detected. From these, 48 genes were identified with (i) more than half having homology to known or hypothetical Haemophilus influenzae genes, (ii) 14 having no ascribed function, and (iii) 4 having very limited or no homology to known genes. The proteins encoded by these genes may, by virtue of their presence on the cell surface, be novel virulence determinants.

* Corresponding author. Mailing address: Molecular Microbiology Group, Department of Biochemistry and Molecular Biology, University College London, Gower St., London WC1E 6BT, United Kingdom. Phone: 0207 504 2242. Fax: 0207 679 7193. E-mail: Ward{at}Biochemistry.ucl.ac.uk.

Infection and Immunity, April 2001, p. 2748-2752, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2748-2752.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ammerman, N. C., Rahman, M. S., Azad, A. F. (2008). Characterization of Sec-Translocon-Dependent Extracytoplasmic Proteins of Rickettsia typhi. J. Bacteriol. 190: 6234-6242 [Abstract] [Full Text]  
  • Mintz, K. P., Moskovitz, J., Wu, H., Fives-Taylor, P. M. (2002). Peptide methionine sulfoxide reductase (MsrA) is not a major virulence determinant for the oral pathogen Actinobacillus actinomycetemcomitans. Microbiology 148: 3695-3703 [Abstract] [Full Text]  
  • Henderson, B. (2002). Oral bacterial disease and the science of cellular conversation. JRSM 95: 77-80 [Full Text]  


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