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Infection and Immunity, April 2001, p. 2753-2756, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2753-2756.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Secretion of Listeriolysin by Brucella suis Inhibits Its Intramacrophagic Replication

Stephan Köhler,^1,* Marion Layssac,¹ Aroem Naroeni,¹ Ivaylo Gentschev,² Michael Rittig,^1, and Jean-Pierre Liautard¹

Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France,¹ and Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften, 97074 Würzburg, Germany²

Received 12 October 2000/Returned for modification 30 November 2000/Accepted 12 January 2001

The introduction into Brucella suis 1330 of a plasmid allowing the heterologous expression of a hybrid cytolysin containing listeriolysin from Listeria monocytogenes, and its export via the Escherichia coli hemolysin secretion pathway, resulted in secretion of active listeriolysin monitored by erythrocyte lysis. In contrast to observations with the nonhemolytic control strain, the phagosomes of infected human monocytes containing the hemolytic B. suis were partially disrupted, and this strain failed to multiply in human macrophage-like cells. These results added strong evidence supporting the proposal that the phagosome of the macrophage was the predominant niche of brucellae in their mammalian hosts.

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^* Corresponding author. Mailing address: INSERM U-431, Université Montpellier II, C.P. 100, Pl. E. Bataillon, 34095 Montpellier, France. Phone: (33) 4 67 14 42 38. Fax: (33) 4 67 14 33 38. E-mail: kohler{at}crit.univ-montp2.fr.

Present address: University of Nottingham Medical School, Queen's Medical Center, Nottingham NG7 2UH, United Kingdom.

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Infection and Immunity, April 2001, p. 2753-2756, Vol. 69, No. 4
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.4.2753-2756.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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