This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Olsen, A. W. Articles by Andersen, P. Search for Related Content PubMed PubMed Citation Articles by Olsen, A. W. Articles by Andersen, P.

Infection and Immunity, May 2001, p. 2773-2778, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2773-2778.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Protection of Mice with a Tuberculosis Subunit Vaccine Based on a Fusion Protein of Antigen 85B and ESAT-6

Anja Weinreich Olsen, Laurens A. H. van Pinxteren, Limei Meng Okkels, Peter Birk Rasmussen, and Peter Andersen^*

Department of TB Immunology, Statens Serum Institute, Copenhagen, Denmark

Received 21 June 2000/Returned for modification 16 August 2000/Accepted 25 January 2001

In this study, we investigated the potential of a tuberculosis subunit vaccine based on fusion proteins of the immunodominant antigens ESAT-6 and antigen 85B. When the fusion proteins were administered to mice in the adjuvant combination dimethyl dioctadecylammonium bromide-monophosphoryl lipid A, a strong dose-dependent immune response was induced to both single components as well as to the fusion proteins. The immune response induced was accompanied by high levels of protective immunity and reached the level of Mycobacterium bovis BCG-induced protection over a broad dose range. The vaccine induced efficient immunological memory, which remained stable 30 weeks postvaccination.

---

^* Corresponding author. Mailing address: Department of TB Immunology, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark. Phone: 45 32 68 34 62. Fax: 45 32 68 30 35. E-mail: pa{at}ssi.dk.

Present address: M&E Biotech, DK-2970, Hørsholm, Denmark.

---

Infection and Immunity, May 2001, p. 2773-2778, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2773-2778.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Dietrich, J., Billeskov, R., Doherty, T. M., Andersen, P. (2007). Synergistic Effect of Bacillus Calmette Guerin and a Tuberculosis Subunit Vaccine in Cationic Liposomes: Increased Immunogenicity and Protection. J. Immunol. 178: 3721-3730 [Abstract] [Full Text]  
• Andersen, C. S., Dietrich, J., Agger, E. M., Lycke, N. Y., Lovgren, K., Andersen, P. (2007). The Combined CTA1-DD/ISCOMs Vector Is an Effective Intranasal Adjuvant for Boosting Prior Mycobacterium bovis BCG Immunity to Mycobacterium tuberculosis. Infect. Immun. 75: 408-416 [Abstract] [Full Text]  
• Dietrich, J., Andersen, C., Rappuoli, R., Doherty, T. M., Jensen, C. G., Andersen, P. (2006). Mucosal Administration of Ag85B-ESAT-6 Protects against Infection with Mycobacterium tuberculosis and Boosts Prior Bacillus Calmette-Guerin Immunity. J. Immunol. 177: 6353-6360 [Abstract] [Full Text]  
• Ebrahim, G. J. (2006). Vaccination against Tuberculosis.. J Trop Pediatr 52: 305-306 [Full Text]  
• Singh, C. R., Moulton, R. A., Armitige, L. Y., Bidani, A., Snuggs, M., Dhandayuthapani, S., Hunter, R. L., Jagannath, C. (2006). Processing and Presentation of a Mycobacterial Antigen 85B Epitope by Murine Macrophages Is Dependent on the Phagosomal Acquisition of Vacuolar Proton ATPase and In Situ Activation of Cathepsin D.. J. Immunol. 177: 3250-3259 [Abstract] [Full Text]  
• Ha, S.-J., Park, S.-H., Kim, H.-J., Kim, S.-C., Kang, H.-J., Lee, E.-G., Kwon, S.-G., Kim, B.-M., Lee, S.-H., Kim, W.-B., Sung, Y.-C., Cho, S.-N. (2006). Enhanced Immunogenicity and Protective Efficacy with the Use of Interleukin-12-Encapsulated Microspheres plus AS01B in Tuberculosis Subunit Vaccination.. Infect. Immun. 74: 4954-4959 [Abstract] [Full Text]  
• Demissie, A., Leyten, E. M. S., Abebe, M., Wassie, L., Aseffa, A., Abate, G., Fletcher, H., Owiafe, P., Hill, P. C., Brookes, R., Rook, G., Zumla, A., Arend, S. M., Klein, M., Ottenhoff, T. H. M., Andersen, P., Doherty, T. M., the VACSEL Study Group, (2006). Recognition of Stage-Specific Mycobacterial Antigens Differentiates between Acute and Latent Infections with Mycobacterium tuberculosis. CVI 13: 179-186 [Abstract] [Full Text]  
• Mukherjee, S., Kashino, S. S., Zhang, Y., Daifalla, N., Rodrigues, V. Jr, Reed, S. G., Campos-Neto, A. (2005). Cloning of the Gene Encoding a Protective Mycobacterium tuberculosis Secreted Protein Detected In Vivo during the Initial Phases of the Infectious Process. J. Immunol. 175: 5298-5305 [Abstract] [Full Text]  
• Doherty, T. M., Andersen, P. (2005). Vaccines for Tuberculosis: Novel Concepts and Recent Progress. Clin. Microbiol. Rev. 18: 687-702 [Abstract] [Full Text]  
• Rosenkrands, I., Agger, E. M., Olsen, A. W., Korsholm, K. S., Andersen, C. S., Jensen, K. T., Andersen, P. (2005). Cationic Liposomes Containing Mycobacterial Lipids: a New Powerful Th1 Adjuvant System. Infect. Immun. 73: 5817-5826 [Abstract] [Full Text]  
• Taylor, J. L., Ordway, D. J., Troudt, J., Gonzalez-Juarrero, M., Basaraba, R. J., Orme, I. M. (2005). Factors Associated with Severe Granulomatous Pneumonia in Mycobacterium tuberculosis-Infected Mice Vaccinated Therapeutically with hsp65 DNA. Infect. Immun. 73: 5189-5193 [Abstract] [Full Text]  
• Martin, C. (2005). The dream of a vaccine against tuberculosis; new vaccines improving or replacing BCG?. Eur Respir J 26: 162-167 [Abstract] [Full Text]  
• Dubnau, E., Chan, J., Mohan, V. P., Smith, I. (2005). Responses of Mycobacterium tuberculosis to Growth in the Mouse Lung. Infect. Immun. 73: 3754-3757 [Abstract] [Full Text]  
• Dietrich, J., Aagaard, C., Leah, R., Olsen, A. W., Stryhn, A., Doherty, T. M., Andersen, P. (2005). Exchanging ESAT6 with TB10.4 in an Ag85B Fusion Molecule-Based Tuberculosis Subunit Vaccine: Efficient Protection and ESAT6-Based Sensitive Monitoring of Vaccine Efficacy. J. Immunol. 174: 6332-6339 [Abstract] [Full Text]  
• Sable, S. B., Verma, I., Behera, D., Khuller, G. K. (2005). Human immune recognition-based multicomponent subunit vaccines against tuberculosis. Eur Respir J 25: 902-910 [Abstract] [Full Text]  
• Wilkinson, K. A., Simsova, M., Scholvinck, E., Sebo, P., Leclerc, C., Vordermeier, H. M., Dickson, S. J., Brown, J. R., Davidson, R. N., Pasvol, G., Levin, M., Wilkinson, R. J. (2005). Efficient Ex Vivo Stimulation of Mycobacterium tuberculosis-Specific T Cells by Genetically Detoxified Bordetella pertussis Adenylate Cyclase Antigen Toxoids. Infect. Immun. 73: 2991-2998 [Abstract] [Full Text]  
• Hovav, A.-H., Fishman, Y., Bercovier, H. (2005). Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants for Mycobacterium tuberculosis Multivalent Acellular Vaccine. Infect. Immun. 73: 250-257 [Abstract] [Full Text]  
• Parra, M., Pickett, T., Delogu, G., Dheenadhayalan, V., Debrie, A.-S., Locht, C., Brennan, M. J. (2004). The Mycobacterial Heparin-Binding Hemagglutinin Is a Protective Antigen in the Mouse Aerosol Challenge Model of Tuberculosis. Infect. Immun. 72: 6799-6805 [Abstract] [Full Text]  
• Mollenkopf, H. J., Grode, L., Mattow, J., Stein, M., Mann, P., Knapp, B., Ulmer, J., Kaufmann, S. H. E. (2004). Application of Mycobacterial Proteomics to Vaccine Design: Improved Protection by Mycobacterium bovis BCG Prime-Rv3407 DNA Boost Vaccination against Tuberculosis. Infect. Immun. 72: 6471-6479 [Abstract] [Full Text]  
• Olsen, A. W., Williams, A., Okkels, L. M., Hatch, G., Andersen, P. (2004). Protective Effect of a Tuberculosis Subunit Vaccine Based on a Fusion of Antigen 85B and ESAT-6 in the Aerosol Guinea Pig Model. Infect. Immun. 72: 6148-6150 [Abstract] [Full Text]  
• Holten-Andersen, L., Doherty, T. M., Korsholm, K. S., Andersen, P. (2004). Combination of the Cationic Surfactant Dimethyl Dioctadecyl Ammonium Bromide and Synthetic Mycobacterial Cord Factor as an Efficient Adjuvant for Tuberculosis Subunit Vaccines. Infect. Immun. 72: 1608-1617 [Abstract] [Full Text]  
• Kohler, A. K., Stone, D. M., Hines, M. T., Byrne, B. A., Alperin, D. C., Norton, L. K., Hines, S. A. (2003). Rhodococcus equi Secreted Antigens Are Immunogenic and Stimulate a Type 1 Recall Response in the Lungs of Horses Immune to R. equi Infection. Infect. Immun. 71: 6329-6337 [Abstract] [Full Text]  
• Yeremeev, V. V., Kondratieva, T. K., Rubakova, E. I., Petrovskaya, S. N., Kazarian, K. A., Telkov, M. V., Biketov, S. F., Kaprelyants, A. S., Apt, A. S. (2003). Proteins of the Rpf Family: Immune Cell Reactivity and Vaccination Efficacy against Tuberculosis in Mice. Infect. Immun. 71: 4789-4794 [Abstract] [Full Text]  
• Hovav, A.-H., Mullerad, J., Davidovitch, L., Fishman, Y., Bigi, F., Cataldi, A., Bercovier, H. (2003). The Mycobacterium tuberculosis Recombinant 27-Kilodalton Lipoprotein Induces a Strong Th1-Type Immune Response Deleterious to Protection. Infect. Immun. 71: 3146-3154 [Abstract] [Full Text]  
• Welsh, M. D., Kennedy, H. E., Smyth, A. J., Girvin, R. M., Andersen, P., Pollock, J. M. (2002). Responses of Bovine WC1+{gamma}{delta} T Cells to Protein and Nonprotein Antigens of Mycobacterium bovis. Infect. Immun. 70: 6114-6120 [Abstract] [Full Text]  
• Repique, C. J., Li, A., Collins, F. M., Morris, S. L. (2002). DNA Immunization in a Mouse Model of Latent Tuberculosis: Effect of DNA Vaccination on Reactivation of Disease and on Reinfection with a Secondary Challenge. Infect. Immun. 70: 3318-3323 [Abstract] [Full Text]  
• D'Souza, S., Rosseels, V., Denis, O., Tanghe, A., De Smet, N., Jurion, F., Palfliet, K., Castiglioni, N., Vanonckelen, A., Wheeler, C., Huygen, K. (2002). Improved Tuberculosis DNA Vaccines by Formulation in Cationic Lipids. Infect. Immun. 70: 3681-3688 [Abstract] [Full Text]  
• Young, D. B, Stewart, G. R (2002). Tuberculosis vaccines. Br Med Bull 62: 73-86 [Abstract] [Full Text]  
• Doherty, T. M., Olsen, A. W., van Pinxteren, L., Andersen, P. (2002). Oral Vaccination with Subunit Vaccines Protects Animals against Aerosol Infection with Mycobacterium tuberculosis. Infect. Immun. 70: 3111-3121 [Abstract] [Full Text]  
• Brandt, L., Feino Cunha, J., Weinreich Olsen, A., Chilima, B., Hirsch, P., Appelberg, R., Andersen, P. (2002). Failure of the Mycobacterium bovis BCG Vaccine: Some Species of Environmental Mycobacteria Block Multiplication of BCG and Induction of Protective Immunity to Tuberculosis. Infect. Immun. 70: 672-678 [Abstract] [Full Text]