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Infection and Immunity, May 2001, p. 2773-2778, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2773-2778.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Protection of Mice with a Tuberculosis Subunit Vaccine Based on a Fusion Protein of Antigen 85B and ESAT-6

Anja Weinreich Olsen, Laurens A. H. van Pinxteren, Limei Meng Okkels, Peter Birk Rasmussen,dagger and Peter Andersen*

Department of TB Immunology, Statens Serum Institute, Copenhagen, Denmark

Received 21 June 2000/Returned for modification 16 August 2000/Accepted 25 January 2001

In this study, we investigated the potential of a tuberculosis subunit vaccine based on fusion proteins of the immunodominant antigens ESAT-6 and antigen 85B. When the fusion proteins were administered to mice in the adjuvant combination dimethyl dioctadecylammonium bromide-monophosphoryl lipid A, a strong dose-dependent immune response was induced to both single components as well as to the fusion proteins. The immune response induced was accompanied by high levels of protective immunity and reached the level of Mycobacterium bovis BCG-induced protection over a broad dose range. The vaccine induced efficient immunological memory, which remained stable 30 weeks postvaccination.

* Corresponding author. Mailing address: Department of TB Immunology, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark. Phone: 45 32 68 34 62. Fax: 45 32 68 30 35. E-mail: pa{at}ssi.dk.

dagger Present address: M&E Biotech, DK-2970, Hørsholm, Denmark.

Infection and Immunity, May 2001, p. 2773-2778, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2773-2778.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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