This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gong, S.
Right arrow Articles by Perry, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gong, S.
Right arrow Articles by Perry, R. D.

 Previous Article  |  Next Article 

Infection and Immunity, May 2001, p. 2829-2837, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.67.5.2829-2837.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of the Yersinia pestis Yfu ABC Inorganic Iron Transport System

Shimei Gong, Scott W. Bearden, Valerie A. Geoffroy, Jacqueline D. Fetherston, and Robert D. Perry*

Department of Microbiology and Immunology, University of Kentucky, Lexington, Kentucky

Received 5 October 2000/Returned for modification 12 January 2001/Accepted 30 January 2001

In Yersinia pestis, the causative agent of plague, two inorganic iron transport systems have been partially characterized. The yersiniabactin (Ybt) system is a siderophore-dependent transport system required for full virulence. Yfe is an ABC transport system that accumulates both iron and manganese. We have identified and cloned a Y. pestis yfuABC operon. The YfuABC system is a member of the cluster of bacterial ABC iron transporters that include Sfu of Serratia, Hit of Haemophilus, and Yfu of Yersinia enterocolitica. The Y. pestis KIM6+ system is most homologous to that in Y. enterocolitica, showing identities of 84% for YfuA (periplasmic binding protein), 87% for YfuB (inner membrane permease), and 75% for YfuC (ATP hydrolase). We constructed a yfuABC promoter-lacZ fusion to examine regulation of transcription. This promoter contains a potential Fur binding sequence and is iron and Fur regulated. Significant expression from the yfuABC promoter occurred during iron-deficient growth conditions. In vitro transcription and translation of a recombinant plasmid encoding yfuABC indicates that YfuABC proteins are expressed. Escherichia coli 1017 (an enterobactin-deficient mutant) carrying this plasmid was able to grow in an iron-restrictive complex medium. We constructed a deletion encompassing the yfuABC promoter and most of yfuA. This mutation was introduced into strains with mutations in Ybt, Yfe, or both systems to examine the role of Yfu in iron acquisition in Y. pestis. Growth of the yfu mutants in a deferrated, defined medium (PMH2) at 26 and 37°C failed to identify a growth or iron transport defect due to the yfu mutation. Fifty percent lethal dose studies in mice did not demonstrate a role for the Yfu system in mammalian virulence.


* Corresponding author. Mailing address: Department of Microbiology and Immunology, MS415 Medical Center, University of Kentucky, Lexington, KY 40536-0298. Phone: (859) 323-6341. Fax: (859) 257-8994. E-mail: rperry{at}pop.uky.edu.


Infection and Immunity, May 2001, p. 2829-2837, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.67.5.2829-2837.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Miller, J. D., Sal, M. S., Schell, M., Whittimore, J. D., Raulston, J. E. (2009). Chlamydia trachomatis YtgA is an iron-binding periplasmic protein induced by iron restriction. Microbiology 155: 2884-2894 [Abstract] [Full Text]  
  • Pujol, C., Klein, K. A., Romanov, G. A., Palmer, L. E., Cirota, C., Zhao, Z., Bliska, J. B. (2009). Yersinia pestis Can Reside in Autophagosomes and Avoid Xenophagy in Murine Macrophages by Preventing Vacuole Acidification. Infect. Immun. 77: 2251-2261 [Abstract] [Full Text]  
  • Douet, V., Expert, D., Barras, F., Py, B. (2009). Erwinia chrysanthemi Iron Metabolism: the Unexpected Implication of the Inner Membrane Platform within the Type II Secretion System. J. Bacteriol. 191: 795-804 [Abstract] [Full Text]  
  • Felek, S., Lawrenz, M. B., Krukonis, E. S. (2008). The Yersinia pestis autotransporter YapC mediates host cell binding, autoaggregation and biofilm formation. Microbiology 154: 1802-1812 [Abstract] [Full Text]  
  • Gao, H., Zhou, D., Li, Y., Guo, Z., Han, Y., Song, Y., Zhai, J., Du, Z., Wang, X., Lu, J., Yang, R. (2008). The Iron-Responsive Fur Regulon in Yersinia pestis. J. Bacteriol. 190: 3063-3075 [Abstract] [Full Text]  
  • Lawlor, M. S., O'Connor, C., Miller, V. L. (2007). Yersiniabactin Is a Virulence Factor for Klebsiella pneumoniae during Pulmonary Infection. Infect. Immun. 75: 1463-1472 [Abstract] [Full Text]  
  • Kirillina, O., Bobrov, A. G., Fetherston, J. D., Perry, R. D. (2006). Hierarchy of Iron Uptake Systems: Yfu and Yiu Are Functional in Yersinia pestis. Infect. Immun. 74: 6171-6178 [Abstract] [Full Text]  
  • Grabenstein, J. P., Fukuto, H. S., Palmer, L. E., Bliska, J. B. (2006). Characterization of Phagosome Trafficking and Identification of PhoP-Regulated Genes Important for Survival of Yersinia pestis in Macrophages. Infect. Immun. 74: 3727-3741 [Abstract] [Full Text]  
  • Sabri, M., Leveille, S., Dozois, C. M. (2006). A SitABCD homologue from an avian pathogenic Escherichia coli strain mediates transport of iron and manganese and resistance to hydrogen peroxide.. Microbiology 152: 745-758 [Abstract] [Full Text]  
  • Lathem, W. W., Crosby, S. D., Miller, V. L., Goldman, W. E. (2005). From The Cover: Progression of primary pneumonic plague: A mouse model of infection, pathology, and bacterial transcriptional activity. Proc. Natl. Acad. Sci. USA 102: 17786-17791 [Abstract] [Full Text]  
  • Shouldice, S. R., McRee, D. E., Dougan, D. R., Tari, L. W., Schryvers, A. B. (2005). Novel Anion-independent Iron Coordination by Members of a Third Class of Bacterial Periplasmic Ferric Ion-binding Proteins. J. Biol. Chem. 280: 5820-5827 [Abstract] [Full Text]  
  • Anderson, D. S., Adhikari, P., Nowalk, A. J., Chen, C. Y., Mietzner, T. A. (2004). The hFbpABC Transporter from Haemophilus influenzae Functions as a Binding-Protein-Dependent ABC Transporter with High Specificity and Affinity for Ferric Iron. J. Bacteriol. 186: 6220-6229 [Abstract] [Full Text]  
  • Perry, R. D., Bobrov, A. G., Kirillina, O., Jones, H. A., Pedersen, L., Abney, J., Fetherston, J. D. (2004). Temperature Regulation of the Hemin Storage (Hms+) Phenotype of Yersinia pestis Is Posttranscriptional. J. Bacteriol. 186: 1638-1647 [Abstract] [Full Text]  
  • Lam, S. L., Kirby, S., Schryvers, A. B. (2003). Foreign signal peptides can constitute a barrier to functional expression of periplasmic proteins in Haemophilus influenzae. Microbiology 149: 3155-3164 [Abstract] [Full Text]  
  • Sheehan, B. J., Bosse, J. T., Beddek, A. J., Rycroft, A. N., Kroll, J. S., Langford, P. R. (2003). Identification of Actinobacillus pleuropneumoniae Genes Important for Survival during Infection in Its Natural Host. Infect. Immun. 71: 3960-3970 [Abstract] [Full Text]  
  • Hazlett, K. R. O., Rusnak, F., Kehres, D. G., Bearden, S. W., La Vake, C. J., La Vake, M. E., Maguire, M. E., Perry, R. D., Radolf, J. D. (2003). The Treponema pallidum tro Operon Encodes a Multiple Metal Transporter, a Zinc-dependent Transcriptional Repressor, and a Semi-autonomously Expressed Phosphoglycerate Mutase. J. Biol. Chem. 278: 20687-20694 [Abstract] [Full Text]  
  • Dhungana, S., Taboy, C. H., Anderson, D. S., Vaughan, K. G., Aisen, P., Mietzner, T. A., Crumbliss, A. L. (2003). Bioinorganic Chemistry Special Feature: The influence of the synergistic anion on iron chelation by ferric binding protein, a bacterial transferrin. Proc. Natl. Acad. Sci. USA 100: 3659-3664 [Abstract] [Full Text]  
  • Brown, J. S., Gilliland, S. M., Ruiz-Albert, J., Holden, D. W. (2002). Characterization of Pit, a Streptococcus pneumoniae Iron Uptake ABC Transporter. Infect. Immun. 70: 4389-4398 [Abstract] [Full Text]  
  • Rossi, M.-S., Fetherston, J. D., Letoffe, S., Carniel, E., Perry, R. D., Ghigo, J.-M. (2001). Identification and Characterization of the Hemophore-Dependent Heme Acquisition System of Yersinia pestis. Infect. Immun. 69: 6707-6717 [Abstract] [Full Text]