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Infection and Immunity, May 2001, p. 2894-2901, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2894-2901.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Salmonella enterica Serovar Typhi Possesses a Unique Repertoire of Fimbrial Gene Sequences

Stacy M. Townsend,1 Naomi E. Kramer,1 Robert Edwards,2 Stephen Baker,3 Nancy Hamlin,3 Mark Simmonds,3 Kim Stevens,3 Stanley Maloy,4 Julian Parkhill,3 Gordon Dougan,5 and Andreas J. Bäumler1,*

Department of Medical Microbiology and Immunology, College of Medicine, Texas A&M University, College Station, Texas 778431; Department of Microbiology and Immunology, University of Tennessee, Memphis, Tennessee 381632; Chemical and Life Sciences Laboratory, University of Illinois, Urbana, Illinois 618014; and The Sanger Centre, The Wellcome Trust Genome Campus, Hixton, Cambridge,3 and Department of Biochemistry, Imperial College, London SW7 2AZ,5 United Kingdom

Received 1 December 2000/Accepted 29 January 2001

Salmonella enterica serotype Typhi differs from nontyphoidal Salmonella serotypes by its strict host adaptation to humans and higher primates. Since fimbriae have been implicated in host adaptation, we investigated whether the serotype Typhi genome contains fimbrial operons which are unique to this pathogen or restricted to typhoidal Salmonella serotypes. This study established for the first time the total number of fimbrial operons present in an individual Salmonella serotype. The serotype Typhi CT18 genome, which has been sequenced by the Typhi Sequencing Group at the Sanger Centre, contained a type IV fimbrial operon, an orthologue of the agf operon, and 12 putative fimbrial operons of the chaperone-usher assembly class. In addition to sef, fim, saf, and tcf, which had been described previously in serotype Typhi, we identified eight new putative chaperone-usher-dependent fimbrial operons, which were termed bcf, sta, stb, ste, std, stc, stg, and sth. Hybridization analysis performed with 16 strains of Salmonella reference collection C and 22 strains of Salmonella reference collection B showed that all eight putative fimbrial operons of serotype Typhi were also present in a number of nontyphoidal Salmonella serotypes. Thus, a simple correlation between host range and the presence of a single fimbrial operon seems at present unlikely. However, the serotype Typhi genome differed from that of all other Salmonella serotypes investigated in that it contained a unique combination of putative fimbrial operons.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, College of Medicine, Texas A&M University System Health Science Center, 407 Reynolds Medical Building, College Station, TX 77843-1114. Phone: (979) 862-7756. Fax: (979) 845-3479. E-mail: abaumler{at}tamu.edu.

Infection and Immunity, May 2001, p. 2894-2901, Vol. 69, No. 5
0019-9567/01/$04.00+0   DOI: 10.1128/IAI.69.5.2894-2901.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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