Salmonella enterica Serovar Typhi Possesses a Unique Repertoire of Fimbrial Gene Sequences

doi:10.1128/IAI.69.5.2894-2901.2001

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Infection and Immunity, May 2001, p. 2894-2901, Vol. 69, No. 5
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.5.2894-2901.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Salmonella enterica Serovar Typhi Possesses a Unique Repertoire of Fimbrial Gene Sequences

Stacy M. Townsend,¹ Naomi E. Kramer,¹ Robert Edwards,² Stephen Baker,³ Nancy Hamlin,³ Mark Simmonds,³ Kim Stevens,³ Stanley Maloy,⁴ Julian Parkhill,³ Gordon Dougan,⁵ and Andreas J. Bäumler¹^,^*

Department of Medical Microbiology and Immunology, College of Medicine, Texas A&M University, College Station, Texas 77843¹; Department of Microbiology and Immunology, University of Tennessee, Memphis, Tennessee 38163²; Chemical and Life Sciences Laboratory, University of Illinois, Urbana, Illinois 61801⁴; and The Sanger Centre, The Wellcome Trust Genome Campus, Hixton, Cambridge,³ and Department of Biochemistry, Imperial College, London SW7 2AZ,⁵ United Kingdom

Received 1 December 2000/Accepted 29 January 2001

Salmonella enterica serotype Typhi differs from nontyphoidal Salmonella serotypes by its strict host adaptation to humansand higher primates. Since fimbriae have been implicated in hostadaptation, we investigated whether the serotype Typhi genomecontains fimbrial operons which are unique to this pathogen orrestricted to typhoidal Salmonella serotypes. This study establishedfor the first time the total number of fimbrial operons presentin an individual Salmonella serotype. The serotype Typhi CT18genome, which has been sequenced by the Typhi Sequencing Groupat the Sanger Centre, contained a type IV fimbrial operon, anorthologue of the agf operon, and 12 putative fimbrial operonsof the chaperone-usher assembly class. In addition to sef, fim,saf, and tcf, which had been described previously in serotypeTyphi, we identified eight new putative chaperone-usher-dependentfimbrial operons, which were termed bcf, sta, stb, ste, std, stc,stg, and sth. Hybridization analysis performed with 16 strainsof Salmonella reference collection C and 22 strains of Salmonellareference collection B showed that all eight putative fimbrialoperons of serotype Typhi were also present in a number of nontyphoidalSalmonella serotypes. Thus, a simple correlation between hostrange and the presence of a single fimbrial operon seems at presentunlikely. However, the serotype Typhi genome differed from thatof all other Salmonella serotypes investigated in that it containeda unique combination of putative fimbrialoperons.

^* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, College of Medicine, Texas A&M UniversitySystem Health Science Center, 407 Reynolds Medical Building, CollegeStation, TX 77843-1114. Phone: (979) 862-7756. Fax: (979) 845-3479.E-mail: abaumler{at}tamu.edu.

Infection and Immunity, May 2001, p. 2894-2901, Vol. 69, No. 5
0019-9567/01/$04.00+0 DOI: 10.1128/IAI.69.5.2894-2901.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

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